Reconstitution of human hypoxia inducible factor HIF-1 in yeast: A simple in vivo system to identify and characterize HIF-1 alpha effectors

Abstract

Hypoxia inducible factor I (HIF-1), the master regulator of hypoxia-activated genes, is involved in many diseases and is a valid drug target. In order to develop a simple and genetically tractable in vivo system for HIF-1 analysis, we tested the inducible expression of both human HIF-1 subunits (HIF-1 alpha and ARNT) in the yeast Saccharomyces cerevisiae and showed the formation of transcriptionally active HIF-1. The use of this system for the identification and characterization of HIF-1 effectors was first validated by showing that two chemical Hsp90 inhibitors, geldanamycin and radicicol, impaired the activity of HIF-1 in yeast. By applying this system in mutant yeast strains. we then identified Hsp90 co-chaperones, which were required for HIF-1 activity. Furthermore, using yeast strains co-expressing truncated forms of HIF-1 alpha with ARNT or both HIF-1 alpha and ARNT, we characterized fragments of HIF-1 alpha that acted as dominant negative mutants and suppressed HIF-1 activity. (c) 2006 Elsevier Inc. All rights reserved.