dc.creator | Braliou, G. G. | en |
dc.creator | Venieris, E. | en |
dc.creator | Kalousi, A. | en |
dc.creator | Simos, G. | en |
dc.date.accessioned | 2015-11-23T10:24:11Z | |
dc.date.available | 2015-11-23T10:24:11Z | |
dc.date.issued | 2006 | |
dc.identifier | 10.1016/j.bbrc.2006.06.043 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | http://hdl.handle.net/11615/26449 | |
dc.description.abstract | Hypoxia inducible factor I (HIF-1), the master regulator of hypoxia-activated genes, is involved in many diseases and is a valid drug target. In order to develop a simple and genetically tractable in vivo system for HIF-1 analysis, we tested the inducible expression of both human HIF-1 subunits (HIF-1 alpha and ARNT) in the yeast Saccharomyces cerevisiae and showed the formation of transcriptionally active HIF-1. The use of this system for the identification and characterization of HIF-1 effectors was first validated by showing that two chemical Hsp90 inhibitors, geldanamycin and radicicol, impaired the activity of HIF-1 in yeast. By applying this system in mutant yeast strains. we then identified Hsp90 co-chaperones, which were required for HIF-1 activity. Furthermore, using yeast strains co-expressing truncated forms of HIF-1 alpha with ARNT or both HIF-1 alpha and ARNT, we characterized fragments of HIF-1 alpha that acted as dominant negative mutants and suppressed HIF-1 activity. (c) 2006 Elsevier Inc. All rights reserved. | en |
dc.source | Biochemical and Biophysical Research Communications | en |
dc.source.uri | <Go to ISI>://WOS:000239016600024 | |
dc.subject | HIF-1 | en |
dc.subject | HIF-1 alpha | en |
dc.subject | ARNT | en |
dc.subject | yeast | en |
dc.subject | hsp90 | en |
dc.subject | geldanamycin | en |
dc.subject | radicicol | en |
dc.subject | sbal | en |
dc.subject | Sti1 | en |
dc.subject | Cpr6 | en |
dc.subject | RECEPTOR NUCLEAR TRANSLOCATOR | en |
dc.subject | SACCHAROMYCES-CEREVISIAE | en |
dc.subject | TRANSCRIPTIONAL | en |
dc.subject | ACTIVATION | en |
dc.subject | HSP90 COCHAPERONE | en |
dc.subject | MODEL SYSTEM | en |
dc.subject | DNA-BINDING | en |
dc.subject | EXPRESSION | en |
dc.subject | PATHWAY | en |
dc.subject | PROTEIN | en |
dc.subject | CHAPERONE | en |
dc.subject | Biochemistry & Molecular Biology | en |
dc.subject | Biophysics | en |
dc.title | Reconstitution of human hypoxia inducible factor HIF-1 in yeast: A simple in vivo system to identify and characterize HIF-1 alpha effectors | en |
dc.type | journalArticle | en |