Cell-specific and roasting-dependent regulation of the Keap1/Nrf2 pathway by coffee extracts
Date
2018Language
en
Sujet
Résumé
Coffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted and the corresponding green one, were investigated for their effect on nuclear factor erythroid 2-related factor 2 (Nrf2) target gene expression in myoblasts and endothelial cells using quantitative PCR. The tested concentrations were non-cytotoxic and led to improved redox cell status, as was evident by increased reduced glutathione (GSH) levels. In both cell lines, the roasted extract upregulated gene expression more readily than its green counterpart leading to increased NAD(P)H quinone dehydrogenase 1 and γ-glutamyl cysteine ligase catalytic subunit, among others. The green extract had a mixed effect on the endothelial cells, while, as regards the myoblasts it caused the downregulation of some Nrf-target genes. Therefore, a potential dose- and roasting-dependent mechanism is proposed in the current study, accounting for coffee's antioxidant activity. © 2018 Spandidos Publications. All rights reserved.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Halophytic herbs of the Mediterranean basin: An alternative approach to health
Petropoulos S.A., Karkanis A., Martins N., Ferreira I.C.F.R. (2018)Wild native species are usually grown under severe and stressful conditions, while a special category includes halophytic species that are tolerant to high salinity levels. Native halophytes are valuable sources of bioactive ... -
Extracts from the mediterranean food plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum enhanced GSH levels and increased Nrf2 expression in human endothelial cells
Stagos D., Balabanos D., Savva S., Skaperda Z., Priftis A., Kerasioti E., Mikropoulou E.V., Vougogiannopoulou K., Mitakou S., Halabalaki M., Kouretas D. (2018)The Mediterranean diet is considered to prevent several diseases. In the present study, the antioxidant properties of six extracts from Mediterranean plant foods were assessed. The extracts' chemical composition analysis ... -
Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells
Goutzourelas, N.; Stagos, D.; Spanidis, Y.; Liosi, M.; Apostolou, A.; Priftis, A.; Haroutounian, S.; Spandidos, D. A.; Tsatsakis, A. M.; Kouretas, D. (2015)The aim of the present study was the assessment of the antioxidant effects of polyphenolic extracts derived from the stems of three Greek grape varieties (Moshomayro, Mavrotragano and Mandilaria) in endothelial (EA.hy926) ...