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Cell-specific and roasting-dependent regulation of the Keap1/Nrf2 pathway by coffee extracts

dc.creatorPriftis A., Angeli-Terzidou A.-E., Veskoukis A.S., Spandidos D.A., Kouretas D.en
dc.date.accessioned2023-01-31T09:50:40Z
dc.date.available2023-01-31T09:50:40Z
dc.date.issued2018
dc.identifier10.3892/mmr.2018.8924
dc.identifier.issn17912997
dc.identifier.urihttp://hdl.handle.net/11615/78362
dc.description.abstractCoffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted and the corresponding green one, were investigated for their effect on nuclear factor erythroid 2-related factor 2 (Nrf2) target gene expression in myoblasts and endothelial cells using quantitative PCR. The tested concentrations were non-cytotoxic and led to improved redox cell status, as was evident by increased reduced glutathione (GSH) levels. In both cell lines, the roasted extract upregulated gene expression more readily than its green counterpart leading to increased NAD(P)H quinone dehydrogenase 1 and γ-glutamyl cysteine ligase catalytic subunit, among others. The green extract had a mixed effect on the endothelial cells, while, as regards the myoblasts it caused the downregulation of some Nrf-target genes. Therefore, a potential dose- and roasting-dependent mechanism is proposed in the current study, accounting for coffee's antioxidant activity. © 2018 Spandidos Publications. All rights reserved.en
dc.language.isoenen
dc.sourceMolecular Medicine Reportsen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85046553254&doi=10.3892%2fmmr.2018.8924&partnerID=40&md5=5d72348a21736886ad9588687f369411
dc.subjectantioxidanten
dc.subjectgamma glutamyl cysteine ligase catalytic subuniten
dc.subjectglutamate cysteine ligaseen
dc.subjectglutathioneen
dc.subjectgreen Coffea arabica extracten
dc.subjectkelch like ECH associated protein 1en
dc.subjectNADPH quinone dehydrogenase 1en
dc.subjectplant extracten
dc.subjectreduced nicotinamide adenine dinucleotide (phosphate) dehydrogenase (quinone)en
dc.subjectroasted Coffea arabica extracten
dc.subjecttranscription factor Nrf2en
dc.subjectunclassified drugen
dc.subjectKEAP1 protein, humanen
dc.subjectkelch like ECH associated protein 1en
dc.subjectplant extracten
dc.subjecttranscription factor Nrf2en
dc.subjectantioxidant activityen
dc.subjectArticleen
dc.subjectC2C12 cell lineen
dc.subjectcoffeeen
dc.subjectcontrolled studyen
dc.subjectdown regulationen
dc.subjectEA.hy 926 cell lineen
dc.subjectgene expressionen
dc.subjectoxidation reduction reactionen
dc.subjectpolymerase chain reactionen
dc.subjectquantitative analysisen
dc.subjectupregulationen
dc.subjectcell lineen
dc.subjectchemistryen
dc.subjectcoffeeen
dc.subjectdrug effecten
dc.subjectgeneticsen
dc.subjecthumanen
dc.subjectmetabolismen
dc.subjectsignal transductionen
dc.subjectCell Lineen
dc.subjectCoffeeen
dc.subjectHumansen
dc.subjectKelch-Like ECH-Associated Protein 1en
dc.subjectNF-E2-Related Factor 2en
dc.subjectPlant Extractsen
dc.subjectSignal Transductionen
dc.subjectSpandidos Publicationsen
dc.titleCell-specific and roasting-dependent regulation of the Keap1/Nrf2 pathway by coffee extractsen
dc.typejournalArticleen


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