Cold shock Y-box protein-1 proteolysis autoregulates its transcriptional activities
Συγγραφέας
Van Roeyen, C. R. C.; Scurt, F. G.; Brandt, S.; Kuhl, V. A.; Martinkus, S.; Djudjaj, S.; Raffetseder, U.; Royer, H. D.; Stefanidis, I.; Dunn, S. E.; Dooley, S.; Weng, H.; Fischer, T.; Lindquist, J. A.; Mertens, P. R.Ημερομηνία
2013Λέξη-κλειδί
Επιτομή
Background: The Y-box protein-1 (YB-1) fulfills pleiotropic functions relating to gene transcription, mRNA processing, and translation. It remains elusive how YB-1 shuttling into the nuclear and cytoplasmic compartments is regulated and whether limited proteolysis by the 20S proteasome releases fragments with distinct function(s) and subcellular distribution(s). Results: To address these questions, mapping of domains responsible for subcellular targeting was performed. Three nuclear localization signals (NLS) were identified. NLS-1 (aa 149-156) and NLS-2 (aa 185-194) correspond to residues with unknown function(s), whereas NLS-3 (aa 276-292) matches with a designated multimerization domain. Nuclear export signal(s) were not identified. Endoproteolytic processing by the 20S proteasome before glycine 220 releases a carboxy-terminal fragment (CTF), which localized to the nucleus, indicating that NLS-3 is operative. Genotoxic stress induced proteolytic cleavage and nuclear translocation of the CTF. Co-expression of the CTF and full-length YB-1 resulted in an abrogated transcriptional activation of the MMP-2 promoter, indicating an autoregulatory inhibitory loop, whereas it fulfilled similar trans-repressive effects on the collagen type I promoter. Conclusion: Compartmentalization of YB-1 protein derivatives is controlled by distinct NLS, one of which targets a proteolytic cleavage product to the nucleus. We propose a model for an autoregulatory negative feedback loop that halts unlimited transcriptional activation. © 2013 van Roeyen et al.; licensee BioMed Central Ltd.
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