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Genetic profiling and volatile oil content of oregano genotypes from greece

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Autor
Papaioannou C., Stefanakis M.K., Batargias C., Kilias G., Anastasopoulos E., Katerinopoulos H.E., Papasotiropoulos V.
Fecha
2020
Language
en
DOI
10.1007/s43450-020-00016-6
Materia
carvacrol
essential oil
microsatellite DNA
monoterpene
para cymene
primer DNA
terpinene
thymol
agar gel electrophoresis
allele
Article
differentiation
DNA isolation
gene amplification
genetic analysis
genetic identification
genetic profile
genetic screening
genotype
Greece
hierarchical clustering
Lamiaceae
marjoram
mass fragmentography
microsatellite marker
oregano
polymerase chain reaction
principal component analysis
Springer Science and Business Media Deutschland GmbH
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Resumen
Several oregano genotypes belonging to Origanum vulgare subsp. hirtum (Link) Ietsw., O. onites L., and O. majorana L., Lamiaceae, from Greece, were genetically and chemically studied. Genetic analysis with microsatellite markers, showed the differentiation of O. onites genotypes from O. vulgare subsp. hirtum and O. majorana ones and revealed several diagnostic alleles which can be used for authentication of the plant raw material. The metabolic profiles of all oregano volatile oils contained carvacrol or thymol as their main volatile component, and others in lower concentration, such as γ-terpinene and p-cymene. Multivariate analyses revealed a clear distinction of the genotypes, based on their chemical composition. According to the Mantel test, there was no correlation between the metabolic and the genetic profile of the oregano genotypes. Molecular identification and chemical characterization contribute to the valorization of oregano material from Greece, which is in great demand, due to its high-quality properties and the various uses in food and pharmaceutical industry. © Sociedade Brasileira de Farmacognosia 2020.
URI
http://hdl.handle.net/11615/77724
Colecciones
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ. [19735]

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