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  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
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Enhancement of the transmesothelial resistance of the parietal sheep peritoneum by epinephrine in vitro: Ussing-type chamber experiments

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Author
Stefanidis, I.; Zarogiannis, S.; Hatzoglou, C.; Liakopoulos, V.; Kourti, P.; Poultsidi, A.; Mertens, P. R.; Gourgoulianis, K.; Molyvdas, P. A.
Date
2005
DOI
10.1111/j.1525-1594.2005.00157.x
Keyword
Epinephrine
Peritoneum
Permeability
Transmesothelial resistance
Ussing-type chamber
adrenalin
bicarbonate
Ringer solution
animal tissue
apical membrane
article
basolateral membrane
controlled study
electric resistance
female
in vitro study
ion transport
male
membrane permeability
mesothelium
nonhuman
peritoneal dialysis
priority journal
sheep
temperature dependence
animal
bioreactor
drug effect
electrochemistry
impedance
instrumentation
methodology
physiology
Ovis aries
Animals
Bioreactors
Electric Impedance
Metadata display
Abstract
The peritoneal mesothelium constitutes an ion transport barrier that is taken advantage of in peritoneal dialysis. The aim of this study was to investigate the effects of epinephrine on the electrical transmesothelial resistance (RTM) of the isolated parietal sheep peritoneum by means of Ussing-type chamber experiments. Intact parietal (diaphragmatic) peritoneal samples were obtained from adult sheep immediately after sacrifice and transferred within 0.5 h to the laboratory in a cooled Krebs-Ringer bicarbonate solution (4°C, pH 7.5), bubbled with 95% O2-5% CO2. A parietal peritoneal planar sheet was mounted in a Ussing-type chamber. Epinephrine (10-7 M) was added to the apical and the basolateral side. The RTM was measured before and serially after the addition of epinephrine for 30 min. As active ion transport is temperature-dependent, all measurements were performed at 37°C. The results were calculated as means with standard errors (x ± SE) of six independent experiments. The control RTM was 20.05 ± 0.61 Ω·cm2. The addition of epinephrine to the basolateral side within 1 min induced an increase of R TM to 21.8 ± 0.45 Ω·cm2, which decreased thereafter progressively to reach control values again after 15 min. A similar effect of epinephrine on the apical side was apparent with a rapid rise of RTM to 22.5 ± 0.66 Ω·cm2 and a subsequent decrease (P < 0.05). A clear association between the RTM and active ion transport was established from previous studies. The results of our study indicate a rapid action of epinephrine on the parietal peritoneum permeability.
URI
http://hdl.handle.net/11615/33427
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