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  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
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  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
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Role of immunohistochemistry for hepatitis D and hepatitis B virus in hepatitis delta

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Author
Kabaçam, G.; Wedemeyer, H.; Savaş, B.; Keskin, O.; Dalekos, G.; Tabak, F.; Idilman, R.; Erhardt, A.; Yalçin, K.; Bozdayi, M. A.; Bozkaya, H.; Manns, M.; Dienes, H.; Yurdaydin, C.; Berg, T.; Böcher, W.; Bohle, H.; Buggisch, P.; Cornberg, M.; Ers̈oz, G.; Feyerabend, S.; Hinrichsen, H.; Manok, M.; Häussinger, D.; Herzog, W.; Hofmann, W. P.; Plein, K.; Hardt, H.; Porst, H.; Mederacke, I.; Rigopoulou, E. I.; Sentürk, H.; Wagner, E. K. V.
Date
2014
DOI
10.1111/liv.12376
Keyword
Chronic delta hepatitis
Delta antigen
HBcAg
HBsAg
Immunohistochemistry
Quantitative surface antigen
alanine aminotransferase
gamma glutamyltransferase
hepatitis B core antigen
hepatitis B surface antigen
virus DNA
biological marker
hepatitis delta antigen
adolescent
adult
alanine aminotransferase blood level
antigen expression
article
cell membrane
controlled study
delta agent hepatitis
disease marker
enzyme immunoassay
female
gamma glutamyl transferase blood level
hepatitis B
Hepatitis B virus
Hepatitis delta virus
human
human tissue
liver
liver injury
major clinical study
male
nonhuman
protein expression
serology
treatment outcome
virus load
age
aged
blood
clinical trial
metabolism
middle aged
multicenter study
nonparametric test
procedures
randomized controlled trial
real time polymerase chain reaction
virology
Age Factors
Alanine Transaminase
Biological Markers
gamma-Glutamyltransferase
Hepatitis B Core Antigens
Hepatitis B Surface Antigens
Hepatitis D, Chronic
Hepatitis delta Antigens
Humans
Real-Time Polymerase Chain Reaction
Statistics, Nonparametric
Metadata display
Abstract
Background & Aims: Immunohistochemical assessment of liver tissue in chronic delta hepatitis (CDH) is underinvestigated. Aim of the study was (i) to assess variables associated with hepatitis D antigen (HDAg), hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) staining in the liver. Methods: Demographic, biochemical and virologic data collected from the HIDIT 1 study were used. HBsAg, HBcAg and HDAg immunohistochemical (IHC) staining was semiquantitatively assessed. Results: Hepatitis D antigen immunohistochemical staining displayed positive correlations with age and alanine aminotransferase (ALT) and negative correlations with serum HBsAg (P = 0.01 for all). HBsAg IHC displayed a negative correlation with gamma glutamyl transferase and positive correlations with serum HBV DNA, serum HBsAg levels and HBeAg serology (P < 0.001, P = 0.02 and P = 0.007 respectively). HBcAg staining was mainly nuclear and displayed negative correlations with serum HBsAg and histologic activity (P = 0.002 and P = 0.02 respectively). Pegylated IFN based treatment led to a decline of all IHC markers, however, these markers had no impact on treatment outcome. Conclusions: These data suggest an association of liver injury with HDAg expression in CDH whereas the negative correlation between HBcAg expression and liver injury and the overall nuclear localization of HBcAg suggest that HBcAg does not contribute to liver injury in CDH. HDV cases with high level of HBV replication, high serum HBsAg levels, HBeAg positivity, that are probably in the earlier stages of disease (low gamma-glutamyl transferase), had a more intense HBsAg staining profile. Overall, the data enforce the importance of HDAg and HBsAg in different phases of CDH infection. © 2013 John Wiley & Sons A/S.
URI
http://hdl.handle.net/11615/28667
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  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ. [9728]

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