In silico transcriptomic analysis of the chloride intracellular channels (Clic) interactome identifies a molecular panel of seven prognostic markers in patients with pancreatic ductal adenocarcinoma
Ημερομηνία
2020Γλώσσα
en
Λέξη-κλειδί
Επιτομή
Background: Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. In this context, the identification of biomarkers regarding the PDAC diagnosis, monitoring, and prognosis is crucial. Objectives: The purpose of the current study was to investigate the differential gene expression profile of the chloride intracellular channel (CLIC) gene family network in patients with PDAC, in order to suggest novel biomarkers. Methods: In silico techniques were used to construct the interactome of the CLIC gene family, identify the differentially expressed genes (DEGs) in PDAC as compared to healthy controls, and evaluate their potential prognostic role. Results: Transcriptomic data of three microarray datasets were included, incorporating 114 tumor and 59 normal pancreatic samples. Twenty DEGs were identified; eight were up-regulated and twelve were downregulated. A molecular signature of seven genes (Chloride Intracellular Channel 1 – CLIC1; Chloride Intracellular Channel 3 – CLIC3; Chloride Intracellular Channel 4 – CLIC4; Gangli-oside Induced Differentiation Associated Protein 1 – GDAP1; Ganglioside Induced Differentiation Associated Protein 1 Like 1 – GDAP1L1; Glutathione S-Transferase Pi 1-GSTP1; Prostaglandin E Synthase 2 – PTGES2) were identified as prognostic markers associated with overall survival. Positive correlations were reported regarding the expression of CLIC1-CLIC3, CLIC4-CLIC5, and CLIC5-CLIC6. Finally, gene set enrichment analysis demonstrated the molecular functions and miRNA families (hsa-‐miR-‐122, hsa-‐miR-‐618, hsa-‐miR-‐425, and hsa-‐miR-‐518) relevant to the seven prognostic markers. Conclusion: These outcomes demonstrate a seven-gene molecular panel that predicts the patients’ prospective survival following pancreatic resection for PDAC. © 2020 Bentham Science Publishers.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Reporting on the Role of miRNAs and Affected Pathways on the Molecular Backbone of Ovarian Insufficiency: A Systematic Review and Critical Analysis Mapping of Future Research
Rapani A., Nikiforaki D., Karagkouni D., Sfakianoudis K., Tsioulou P., Grigoriadis S., Maziotis E., Pantou A., Voutsina A., Pantou A., Koutsilieris M., Hatzigeorgiou A., Pantos K., Simopoulou M. (2021)Ovarian insufficiency is identified as a perplexing entity in the long list of pathologies impairing fertility dynamics. The three distinct classifications of ovarian insufficiency are poor ovarian response, premature ... -
Parkinson's disease and pesticides: Are microRNAs the missing link?
Aloizou A.-M., Siokas V., Sapouni E.-M., Sita N., Liampas I., Brotis A.G., Rakitskii V.N., Burykina T.I., Aschner M., Bogdanos D.P., Tsatsakis A., Hadjigeorgiou G.M., Dardiotis E. (2020)Parkinson's disease (PD) is a common neurodegenerative disorder that leads to significant morbidity and decline in the quality of life. It develops due to loss of dopaminergic neurons in the substantia nigra pars compacta, ... -
Identification of neoplasm-specific signatures of miRNA interactions by employing a systems biology approach
Bonab R.A., Asfa S., Kontou P., Karakülah G., Pavlopoulou A. (2022)MicroRNAs represent major regulatory components of the disease epigenome and they constitute powerful biomarkers for the accurate diagnosis and prognosis of various diseases, including cancers. The advent of high-throughput ...