dc.creator | Efrose R.C., Rosu C.M., Stedel C., Stefan A., Sirbu C., Gorgan L.D., Labrou N.E., Flemetakis E. | en |
dc.date.accessioned | 2023-01-31T07:37:06Z | |
dc.date.available | 2023-01-31T07:37:06Z | |
dc.date.issued | 2018 | |
dc.identifier | 10.1007/s10482-017-0934-3 | |
dc.identifier.issn | 00036072 | |
dc.identifier.uri | http://hdl.handle.net/11615/71273 | |
dc.description.abstract | The symbiotic nitrogen fixing legumes play an essential role in sustainable agriculture. White clover (Trifolium repens L.) is one of the most valuable perennial legumes in pastures and meadows of temperate regions. Despite its great agriculture and economic importance, there is no detailed available information on phylogenetic assignation and characterization of rhizobia associated with native white clover plants in South-Eastern Europe. In the present work, the diversity of indigenous white clover rhizobia originating in 11 different natural ecosystems in North-Eastern Romania were assessed by a polyphasic approach. Initial grouping showed that, 73 rhizobial isolates, representing seven distinct phenons were distributed into 12 genotypes, indicating a wide phenotypic and genotypic diversity among the isolates. To clarify their phylogeny, 44 representative strains were used in sequence analysis of 16S rRNA gene and IGS fragments, three housekeeping genes (atpD, glnII and recA) and two symbiosis-related genes (nodA and nifH). Multilocus sequence analysis (MLSA) phylogeny based on concatenated housekeeping genes delineated the clover isolates into five putative genospecies. Despite their diverse chromosomal backgrounds, test strains shared highly similar symbiotic genes closely related to Rhizobium leguminosarum biovar trifolii. Phylogenies inferred from housekeeping genes were incongruent with those of symbiotic genes, probably due to occurrence of lateral transfer events among native strains. This is the first polyphasic taxonomic study to report on the MLSA-based phylogenetic diversity of indigenous rhizobia nodulating white clover plants grown in various soil types in South-Eastern Europe. Our results provide valuable taxonomic data on native clover rhizobia and may increase the pool of genetic material to be used as biofertilizers. © 2017, Springer International Publishing AG. | en |
dc.language.iso | en | en |
dc.source | Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029410303&doi=10.1007%2fs10482-017-0934-3&partnerID=40&md5=9fbbfb78d01dc0f3e7f07f8cf34aecc4 | |
dc.subject | RNA 16S | en |
dc.subject | RNA 23S | en |
dc.subject | Article | en |
dc.subject | atpD gene | en |
dc.subject | bacterial gene | en |
dc.subject | bacterial strain | en |
dc.subject | bacterium isolate | en |
dc.subject | controlled study | en |
dc.subject | ecosystem | en |
dc.subject | genetic variability | en |
dc.subject | genotype | en |
dc.subject | glnII gene | en |
dc.subject | housekeeping gene | en |
dc.subject | multilocus sequence typing | en |
dc.subject | native species | en |
dc.subject | nifH gene | en |
dc.subject | nodA gene | en |
dc.subject | nodulation | en |
dc.subject | nonhuman | en |
dc.subject | nucleotide sequence | en |
dc.subject | phenotype | en |
dc.subject | phylogeny | en |
dc.subject | polymerase chain reaction | en |
dc.subject | priority journal | en |
dc.subject | RecA gene | en |
dc.subject | restriction fragment length polymorphism | en |
dc.subject | Rhizobiaceae | en |
dc.subject | Rhizobium leguminosarum | en |
dc.subject | Romania | en |
dc.subject | sequence analysis | en |
dc.subject | symbiosis | en |
dc.subject | taxonomy | en |
dc.subject | unindexed sequence | en |
dc.subject | white clover | en |
dc.subject | bacterial genome | en |
dc.subject | biodiversity | en |
dc.subject | classification | en |
dc.subject | clover | en |
dc.subject | essential gene | en |
dc.subject | genetic variation | en |
dc.subject | genetics | en |
dc.subject | genomics | en |
dc.subject | microbiology | en |
dc.subject | molecular typing | en |
dc.subject | procedures | en |
dc.subject | Rhizobium leguminosarum | en |
dc.subject | Biodiversity | en |
dc.subject | Genes, Bacterial | en |
dc.subject | Genes, Essential | en |
dc.subject | Genetic Variation | en |
dc.subject | Genome, Bacterial | en |
dc.subject | Genomics | en |
dc.subject | Molecular Typing | en |
dc.subject | Multilocus Sequence Typing | en |
dc.subject | Phenotype | en |
dc.subject | Phylogeny | en |
dc.subject | Rhizobium leguminosarum | en |
dc.subject | Trifolium | en |
dc.subject | Springer Netherlands | en |
dc.title | Molecular diversity and phylogeny of indigenous Rhizobium leguminosarum strains associated with Trifolium repens plants in Romania | en |
dc.type | journalArticle | en |