Lincomycin resistance gene lnu(D) in Streptococcus uberis
AuthorPetinaki, E.; Guérin-Faublée, V.; Pichereau, V.; Villers, C.; Achard, A.; Malbruny, B.; Leclercq, R.
Streptococcus uberis UCN 42, isolated from a case of bovine mastitis, was intermediately resistant to lincomycin (MIC = 2 μg/ml) while remaining susceptible to clindamycin (MIC = 0.06 μg/ml) and erythromycin. A 1.1-kb SacI fragment was cloned from S. uberis UCN 42 total DNA on plasmid pUC 18 and introduced into Escherichia coli AG100A, where it conferred resistance to both clindamycin and lincomycin. The sequence analysis of the fragment showed the presence of a new gene, named lnu(D), that encoded a 164-amino-acid protein with 53% identity with Lnu(C) previously reported to occur in Streptococcus agalactiae:Crude lysates of E. coli AG100A containing the cloned lnu(D) gene inactivated lincomycin and clindamycin in the presence of ATP and MgCl 2. Mass spectrometry experiments demonstrated that the lnu(D) enzyme catalyzed adenylylation of clindamycin. A domain conserved in deduced sequences of lincosamide O-nucleotidyltransferases Lnu(A), Lnu(C), LinAN2, and Lin(D) and in the aminoglycoside nucleotidyltransferase ANT(2″) was identified. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Showing items related by title, author, creator and subject.
Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1 Noutsios, G. T.; Papi, R. M.; Ekateriniadou, L. V.; Minas, A.; Kyriakidis, D. A. (2012)In the present study forty-four Greek endemic strains of Br. melitensis and three reference strains were genotyped by Multi locus Variable Number Tandem Repeat (ML-VNTR) analysis based on an eight-base pair tandem repeat ...
Direct detection of Cardiobacterium hominis in serum from a patient with infective endocarditis by broad-range bacterial PCR Gatselis, N.; Malli, E.; Papadamou, G.; Petinaki, E.; Dalekos, G. N. (2006)Bacterial DNA was detected directly to the serum of a patient with endocarditis by broad-range 16S rRNA PCR followed by sequencing and analysis of the results by the BLAST search. Using these methods, Cardiobacterium hominis ...
Korencic, D.; Ahel, I.; Schelert, J.; Sacher, M.; Ruan, B.; Stathopoulos, C.; Blum, P.; Ibba, M.; Söll, D. (2004)Threonyl-tRNA synthetase (ThrRS) participates in protein synthesis quality control by selectively editing the misacylated species Ser-tRNAThr. In bacteria and eukaryotes the editing function of ThrRS resides in a highly ...