Evaluation of a Direct Immunofluorescent Assay and/or Conjunctival Cytology for Detection of Canine Distemper Virus Antigen
dc.creator | Athanasiou L.V., Kantere M.C., Kyriakis C.S., Pardali D., Adamama Moraitou K., Polizopoulou Z.S. | en |
dc.date.accessioned | 2023-01-31T07:33:58Z | |
dc.date.available | 2023-01-31T07:33:58Z | |
dc.date.issued | 2018 | |
dc.identifier | 10.1089/vim.2017.0101 | |
dc.identifier.issn | 08828245 | |
dc.identifier.uri | http://hdl.handle.net/11615/70914 | |
dc.description.abstract | Canine distemper is a common and potentially lethal multisystemic disease caused by the Canine distemper virus (CDV). We evaluated the diagnostic performance of direct immunofluorescent assay (FA) and cytology to detect CDV antigen in conjunctival cells compared with an established polymerase chain reaction (PCR) detection assay used as a gold standard for CDV diagnosis. Samples were collected from 57 young dogs presenting with central nervous system signs compatible with distemper disease. Exfoliative epithelial cells were collected from the right and left conjunctiva of each animal using nylon-bristled cytobrushes for cytology and cotton swabs for FA and PCR. For the direct FA, samples were stained with anti-CDV polyclonal antiserum conjugated to fluorescein isothiocyanate and imaged using a fluorescent microscope. Out of 57 dogs tested, 19 were PCR positive (15 positive in direct FA and 4 positive in cytology, including one that was negative by PCR), whereas 37 dogs were negative in all methods. A good agreement was observed between the FA and PCR, with a κ-value of 0.833 (95% CI: 0.678-0.989). Meanwhile, there was poor agreement between cytology and PCR (κ-value of 0.164; 95% CI: -0.045 to 0.373) and a fair agreement between FA and cytology (κ-value of 0.231; 95% CI: -0.026 to 0.487). Our results indicated a poor performance of cytology for the detection of CDV antigen. In contrast, FA is a 100% specific and an adequately sensitive assay (sensitivity: 78.95%, negative likelihood ratio: 0.21, 95% CI: 0.09-0.50) for antemortem diagnosis of canine distemper. © 2018 Mary Ann Liebert, Inc. | en |
dc.language.iso | en | en |
dc.source | Viral Immunology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045515003&doi=10.1089%2fvim.2017.0101&partnerID=40&md5=e3e56e5da55b8289ce434035c36145a7 | |
dc.subject | fluorescein isothiocyanate | en |
dc.subject | nylon | en |
dc.subject | polyclonal antiserum | en |
dc.subject | virus antigen | en |
dc.subject | fluorescent dye | en |
dc.subject | virus antibody | en |
dc.subject | virus antigen | en |
dc.subject | animal cell | en |
dc.subject | antigen detection | en |
dc.subject | Article | en |
dc.subject | canine distemper | en |
dc.subject | conjunctiva | en |
dc.subject | controlled study | en |
dc.subject | cytology | en |
dc.subject | epithelium cell | en |
dc.subject | immunofluorescence test | en |
dc.subject | nonhuman | en |
dc.subject | polymerase chain reaction | en |
dc.subject | puppy | en |
dc.subject | sensitivity and specificity | en |
dc.subject | animal | en |
dc.subject | canine distemper | en |
dc.subject | Canine distemper virus | en |
dc.subject | conjunctiva | en |
dc.subject | cytology | en |
dc.subject | direct fluorescent antibody technique | en |
dc.subject | dog | en |
dc.subject | evaluation study | en |
dc.subject | fluorescence microscopy | en |
dc.subject | immunology | en |
dc.subject | procedures | en |
dc.subject | staining | en |
dc.subject | virology | en |
dc.subject | Animals | en |
dc.subject | Antibodies, Viral | en |
dc.subject | Antigens, Viral | en |
dc.subject | Conjunctiva | en |
dc.subject | Cytological Techniques | en |
dc.subject | Distemper | en |
dc.subject | Distemper Virus, Canine | en |
dc.subject | Dogs | en |
dc.subject | Fluorescent Antibody Technique, Direct | en |
dc.subject | Fluorescent Dyes | en |
dc.subject | Microscopy, Fluorescence | en |
dc.subject | Polymerase Chain Reaction | en |
dc.subject | Sensitivity and Specificity | en |
dc.subject | Staining and Labeling | en |
dc.subject | Mary Ann Liebert Inc. | en |
dc.title | Evaluation of a Direct Immunofluorescent Assay and/or Conjunctival Cytology for Detection of Canine Distemper Virus Antigen | en |
dc.type | journalArticle | en |
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