Μελέτη της έκφρασης του γονιδίου της ντεκορίνης σε ισχαιμικό-υποξικό εγκέφαλο επιμυών
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Auteur
Καραμόσχου, ΈλληSupervisor name
Κριτής, Αριστείδης Α.
Date
2003Language
el
Access
free
Résumé
In the present study we investigate the expression of decorin gene in neonatal rat
brain during ichemic and hypoxic conditions. Decorin is a protein molecule which
belongs to the family of small rich in leucine proteoglycans (SLRPs) and it is present
in several cells through maturation process. Decorin interacts with important factors
that influence the cellular cycle as TGF-β (transformer growth factor β) and the
receptor of EGF (epidermic growth factor) and with elements of extra cellular matrix
as collagen. Those properties of decorin conduct us to the suspicion that it plays an
important role in the control of cellular cycle and the process of maturation and
proliferation.
For the experiment procedure we used seven-day-old wistar rat pups that were
exposed in hypoxic conditions (8% oxygen -92% nitrogen mixture for 60 min), two
or three hours after permenant occlusion of their left common carotid. We isolated
RNA from the brain of rats who were sacrificed at Oh, 2h, 24h, 48h, 72h and 7 days
after the ichemic and hypoxic conditions. The RNA was subjected to reserve
transcription and competitive PCR amplification under standard conditions including
b-actin as internal control for gene expression.
Our results demonstrated an initial diminution both in the right and the left
hemisphere of decorin gene expression due in the cell death during ichemia / hypoxia
and a tension of return in normal levels from 48h. After seven days decorin gene
expression seems to increase up to normal levels. This results support previous
studies, which correlate the increased levels of decorin during angiogenesis, with the
formation of capillaries.
Academic publisher
Πανεπιστήμιο Θεσσαλίας. Πρόγραμμα Σπουδών Επιλογής. Τμήμα Ιατρικής Βιοχημείας.