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dc.creatorPapatheodorou, L. K.en
dc.creatorMalizos, K. N.en
dc.creatorPoultsides, L. A.en
dc.creatorHantes, M. E.en
dc.creatorGrafanaki, K.en
dc.creatorGiannouli, S.en
dc.creatorIoannou, M. G.en
dc.creatorKoukoulis, G. K.en
dc.creatorProtopappas, V. C.en
dc.creatorFotiadis, D. I.en
dc.creatorStathopoulos, C.en
dc.date.accessioned2015-11-23T10:44:30Z
dc.date.available2015-11-23T10:44:30Z
dc.date.issued2009
dc.identifier10.1016/j.ultrasmedbio.2008.07.003
dc.identifier.issn0301-5629
dc.identifier.urihttp://hdl.handle.net/11615/31954
dc.description.abstractThe present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) on the healing at tendon graft-bone interface, in molecular and histological level. The anterior cruciate ligament (ACL) in both knees of 52 New Zealand White rabbits was excised and replaced with the long digital extensor. A custom-made ultrasound transducer was implanted onto the medial tibial condyle, adjacent to the surface of the bone tunnel at both knees of the rabbits. The LiUS-treated right knees received 200-mu s bursts of 1 MHz sine waves at a pulse repetition rate of 1 kHz and with 30 MW/cm(2) spatial-average temporal-average intensity for 20 min daily (study group), while the left knee received no LiUS (control group). Thirty-six rabbits were used to perform semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis from both study and control groups for transforming growth factor-beta 1 (TGF-beta 1), biglycan and collagen I. RT-PCR products showed statistically significant upregulation of biglycan and collagen I gene expression in the study group, while TGF-beta 1 gene expression exhibited a bimodal profile. Histological examination performed in 16 rabbits from both groups supported the findings of the molecular analysis, indicating a faster healing rate and a more efficient ligamentization process after ultrasound treatment. These findings suggest that transosseous application of LiUS enhances the healing rate of the tendon graft-bone interface, possibly by affecting the expression levels of genes significant for the tendon to bone healing process. (E-mail: malizos@med.uth.gr) (c) 2009 World Federation for Ultrasound in Medicine & Biology.en
dc.sourceUltrasound in Medicine and Biologyen
dc.source.uri<Go to ISI>://WOS:000264977300007
dc.subjectLow-intensity ultrasound (LiUS)en
dc.subjectTendon graft-bone interface healingen
dc.subjectTGF-beta 1en
dc.subjectBiglycanen
dc.subjectCollagen type Ien
dc.subjectAnterior cruciate ligament (ACL)en
dc.subjectANTERIOR CRUCIATE LIGAMENTen
dc.subjectMESSENGER-RNA LEVELSen
dc.subjectPULSED ULTRASOUNDen
dc.subjectGROWTH-FACTORSen
dc.subjectPATELLAR TENDONen
dc.subjectTRANSFORMING GROWTH-FACTOR-BETA-1en
dc.subjectAUTOGENOUS PATELLARen
dc.subjectCOLLAGEN-SYNTHESISen
dc.subjectHUMAN-FIBROBLASTSen
dc.subjectMATRIXen
dc.subjectSYNTHESISen
dc.subjectAcousticsen
dc.subjectRadiology, Nuclear Medicine & Medical Imagingen
dc.titleEFFECT OF TRANSOSSEOUS APPLICATION OF LOW-INTENSITY ULTRASOUND AT THE TENDON GRAFT-BONE INTERFACE HEALING: GENE EXPRESSION AND HISTOLOGICAL ANALYSIS IN RABBITSen
dc.typejournalArticleen


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