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  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
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  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
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EFFECT OF TRANSOSSEOUS APPLICATION OF LOW-INTENSITY ULTRASOUND AT THE TENDON GRAFT-BONE INTERFACE HEALING: GENE EXPRESSION AND HISTOLOGICAL ANALYSIS IN RABBITS

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Author
Papatheodorou, L. K.; Malizos, K. N.; Poultsides, L. A.; Hantes, M. E.; Grafanaki, K.; Giannouli, S.; Ioannou, M. G.; Koukoulis, G. K.; Protopappas, V. C.; Fotiadis, D. I.; Stathopoulos, C.
Date
2009
DOI
10.1016/j.ultrasmedbio.2008.07.003
Keyword
Low-intensity ultrasound (LiUS)
Tendon graft-bone interface healing
TGF-beta 1
Biglycan
Collagen type I
Anterior cruciate ligament (ACL)
ANTERIOR CRUCIATE LIGAMENT
MESSENGER-RNA LEVELS
PULSED ULTRASOUND
GROWTH-FACTORS
PATELLAR TENDON
TRANSFORMING GROWTH-FACTOR-BETA-1
AUTOGENOUS PATELLAR
COLLAGEN-SYNTHESIS
HUMAN-FIBROBLASTS
MATRIX
SYNTHESIS
Acoustics
Radiology, Nuclear Medicine & Medical Imaging
Metadata display
Abstract
The present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) on the healing at tendon graft-bone interface, in molecular and histological level. The anterior cruciate ligament (ACL) in both knees of 52 New Zealand White rabbits was excised and replaced with the long digital extensor. A custom-made ultrasound transducer was implanted onto the medial tibial condyle, adjacent to the surface of the bone tunnel at both knees of the rabbits. The LiUS-treated right knees received 200-mu s bursts of 1 MHz sine waves at a pulse repetition rate of 1 kHz and with 30 MW/cm(2) spatial-average temporal-average intensity for 20 min daily (study group), while the left knee received no LiUS (control group). Thirty-six rabbits were used to perform semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis from both study and control groups for transforming growth factor-beta 1 (TGF-beta 1), biglycan and collagen I. RT-PCR products showed statistically significant upregulation of biglycan and collagen I gene expression in the study group, while TGF-beta 1 gene expression exhibited a bimodal profile. Histological examination performed in 16 rabbits from both groups supported the findings of the molecular analysis, indicating a faster healing rate and a more efficient ligamentization process after ultrasound treatment. These findings suggest that transosseous application of LiUS enhances the healing rate of the tendon graft-bone interface, possibly by affecting the expression levels of genes significant for the tendon to bone healing process. (E-mail: malizos@med.uth.gr) (c) 2009 World Federation for Ultrasound in Medicine & Biology.
URI
http://hdl.handle.net/11615/31954
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