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dc.contributor.advisorΜόσιαλος, Δημήτρηςel
dc.creatorΣακελλάρης, Βίκτωρel
dc.date.accessioned2015-01-05T21:42:40Z
dc.date.available2015-01-05T21:42:40Z
dc.date.issued2010
dc.identifier.other8172
dc.identifier.urihttp://hdl.handle.net/11615/305en
dc.description.abstractPseudomonas aeruginosa is one of the most common gram-negative hospitalacquired pathogens. This organism shows a remarkable resistance to antibiotics, mostly because of acquisition of resistance genes e.g., genes for beta-lactamases, combined with over-expression of efflux pumps and/or decreased expression of porins. The most common beta-lactamases are IMP and VIM type MBL, as well as OXA-ESBLS and give to the bacteria high resistance to b-lactam antibiotics, especially carbapenems. The genes for VIM and IMP enzymes, like those for OXA-ESBLs, are transferable as most are found as gene cassettes located primarily on integrons, sometimes within transposons or on plasmids, a condition that provides a wide potential for expression and dissemination in gram-negative pathogens. Since the first finding of IMP] metallo-B-lactamase in a clinical isolate of Pseudomonas aeruginosa in Japan in 1988, IMP-type enzymes are spread worldwide, increasing in two decades the proportion of P. aeruginosa isolates demonstrating resistance to imipenem, due to IMPmbls? from 13% to 20%. Currently have indentified 26 carbapenem-hydrolyzing enzymes of the IMP-type. In this study we present the discovery of a new IMP type MBL from 6 P. aeruginosa clinical strains. This novel IMP-type MBL is highly divergent from IMPi, possessing 44 different amino acids, whereas the most divergent till now IMP-type MBL, IMP12, has only 36 different amino acids from IMP]. This new IMP-MBLs was found in a class 1 integron next to ΟΧʼ35 gene. The next objective will be the biochemical characterization of this new IMP metallo-fl-lactamase.en
dc.language.isoel_GRen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subject.otherΓΟΝΙΔΙΑ -- ΑΝΑΛΥΣΗel
dc.subject.otherΑΝΤΙΒΙΟΤΙΚΑel
dc.subject.otherGRAM ΑΡΝΗΤΙΚΑ ΒΑΚΤΗΡΙΑel
dc.titleΜελέτη του γενετικού μηχανισμού αντοχής στις καρβαπενέμες σε κλινικά στελέχη Pseudomonas aeruginosael
dc.typebachelorThesisen
heal.recordProviderΠανεπιστήμιο Θεσσαλίας - Βιβλιοθήκη και Κέντρο Πληροφόρησηςel
heal.academicPublisherΠανεπιστήμιο Θεσσαλίας. Σχολή Επιστημών Υγείας. Τμήμα Βιοχημείας και Βιοτεχνολογίας.el
heal.academicPublisherIDuthen
heal.fullTextAvailabilitytrueen
dc.rights.accessRightsfreeen


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International