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dc.creatorKarligiotou, E.en
dc.creatorKollia, P.en
dc.creatorKallitsaris, A.en
dc.creatorMessinis, I. E.en
dc.date.accessioned2015-11-23T10:33:46Z
dc.date.available2015-11-23T10:33:46Z
dc.date.issued2006
dc.identifier10.1093/humrep/dei374
dc.identifier.issn2681161
dc.identifier.urihttp://hdl.handle.net/11615/29157
dc.description.abstractIntroduction: According to previous studies, gonadotrophin surge-attenuating factor (GnSAF), which is assumed to be produced in human granulosa cells, has a homology with the carboxyl terminal of the human serum albumin (HSA) protein. In an attempt to validate these findings, whole or partial expression of the HSA gene was studied by RT-PCR analysis in human granulosa cells from women undergoing IVF treatment. Methods: RT-PCR analysis of HSA RNA transcripts in luteinized granulosa cells was done in order to investigate the possible expression of the HSA gene. To ensure the specificity of PCR products, restriction enzyme and sequence analysis were performed. Western blot analysis was carried out to detect the possible expression of the albumin gene in granulosa cells. Results: RT-PCR analysis and sequencing analysis of cDNA from granulosa cells revealed bands identical with those from the positive control for the amino as well as the carboxyl terminal corresponding to HSA gene at the cytoplasmic level. Conclusion: We have demonstrated that human granulosa cells express the carboxyl and amino terminal part of the HSA gene in levels comparable to those found in human hepatocytes. It is suggested that the coding gene for GnSAF may be a result of an alternative expression of HSA gene. © 2006 Oxford University Press.en
dc.source.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-33644862054&partnerID=40&md5=d05c2ec004b0be4d230df97164416277
dc.subjectAlbuminen
dc.subjectGnSAFen
dc.subjectGranulosa cellsen
dc.subjectOvaryen
dc.subjectamino aciden
dc.subjectcomplementary DNAen
dc.subjectgonadotropin surge attenuating factoren
dc.subjecthuman serum albuminen
dc.subjectmessenger RNAen
dc.subjectadulten
dc.subjectamino terminal sequenceen
dc.subjectarticleen
dc.subjectcarboxy terminal sequenceen
dc.subjectcellular distributionen
dc.subjectcontrolled studyen
dc.subjectcorrelation analysisen
dc.subjectcytoplasmen
dc.subjectfemaleen
dc.subjectfertilization in vitroen
dc.subjectgenetic transcriptionen
dc.subjectgranulosa cellen
dc.subjecthumanen
dc.subjecthuman cellen
dc.subjectliver cellen
dc.subjectluteinizationen
dc.subjectprotein expressionen
dc.subjectprotein localizationen
dc.subjectrestriction mappingen
dc.subjectreverse transcription polymerase chain reactionen
dc.subjectsequence analysisen
dc.subjectvalidation processen
dc.subjectWestern blottingen
dc.subjectAmino Acid Sequenceen
dc.subjectBase Sequenceen
dc.subjectDNA Primersen
dc.subjectGonadal Hormonesen
dc.subjectHumansen
dc.subjectPeptide Fragmentsen
dc.subjectProteinsen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectRNA, Messengeren
dc.subjectSerum Albuminen
dc.titleExpression of human serum albumin (HSA) mRNA in human granulosa cells: Potential correlation of the 95 amino acid long carboxyl terminal of HSA to gonadotrophin surge-attenuating factoren
dc.typejournalArticleen


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