dc.creator | Vliora M., Grillo E., Corsini M., Ravelli C., Nintou E., Karligiotou E., Flouris A.D., Mitola S. | en |
dc.date.accessioned | 2023-01-31T11:37:04Z | |
dc.date.available | 2023-01-31T11:37:04Z | |
dc.date.issued | 2022 | |
dc.identifier | 10.1016/j.bbagen.2022.130085 | |
dc.identifier.issn | 03044165 | |
dc.identifier.uri | http://hdl.handle.net/11615/80696 | |
dc.description.abstract | Background: Adipose tissue plays a pivotal role in the development and progression of the metabolic syndrome which along with its complications is an epidemic of the 21st century. Irisin is an adipo-myokine secreted mainly by skeletal muscle and targeting, among others, adipose tissue. In brown adipose tissue it upregulates uncoupling protein-1 (UCP1) which is responsible for mitochondrial non-shivering thermogenesis. Methods: Here we analyzed the effects of irisin on the metabolic activity of 3T3-L1 derived adipocytes through a mitochondrial flux assay. We also assessed the effects of irisin on the intracellular signaling through Western Blot. Finally, the gene expression of ucp1 and lipolytic genes was examined through RT-qPCR. Results: Irisin affects mitochondrial respiration and lipolysis in a time-dependent manner through the regulation of PI3K-AKT pathway. Irisin also induces the expression of UCP1 and the regulation of NF-κB, and CREB and ERK pathways. Conclusion: Our data supports the role of irisin in the induction of non-shivering thermogenesis, the regulation of energy expenditure and lipolysis in adipocytes. General significance: Irisin may be an attractive therapeutic target in the treatment of obesity and related metabolic disorders. © 2022 | en |
dc.language.iso | en | en |
dc.source | Biochimica et Biophysica Acta - General Subjects | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122618109&doi=10.1016%2fj.bbagen.2022.130085&partnerID=40&md5=1f8b56d3f112998953a6fa3b63b0edf4 | |
dc.subject | cyclic AMP responsive element binding protein | en |
dc.subject | immunoglobulin enhancer binding protein | en |
dc.subject | irisin | en |
dc.subject | mitogen activated protein kinase | en |
dc.subject | uncoupling protein 1 | en |
dc.subject | fibronectin | en |
dc.subject | phosphatidylinositol 3 kinase | en |
dc.subject | transcription factor | en |
dc.subject | 3T3-L1 cell line | en |
dc.subject | adipose tissue | en |
dc.subject | animal cell | en |
dc.subject | Article | en |
dc.subject | cell metabolism | en |
dc.subject | controlled study | en |
dc.subject | drug efficacy | en |
dc.subject | energy expenditure | en |
dc.subject | gene expression regulation | en |
dc.subject | intracellular signaling | en |
dc.subject | lipolysis | en |
dc.subject | mitochondrial dynamics | en |
dc.subject | mitochondrial respiration | en |
dc.subject | mouse | en |
dc.subject | nonhuman | en |
dc.subject | Pi3K/Akt signaling | en |
dc.subject | real time polymerase chain reaction | en |
dc.subject | thermogenesis | en |
dc.subject | ucp1 gene | en |
dc.subject | Western blotting | en |
dc.subject | 3T3-L1 cell line | en |
dc.subject | adipocyte | en |
dc.subject | animal | en |
dc.subject | brown adipose tissue | en |
dc.subject | genetics | en |
dc.subject | metabolism | en |
dc.subject | thermogenesis | en |
dc.subject | 3T3-L1 Cells | en |
dc.subject | Adipocytes | en |
dc.subject | Adipose Tissue, Brown | en |
dc.subject | Animals | en |
dc.subject | Fibronectins | en |
dc.subject | Lipolysis | en |
dc.subject | Mice | en |
dc.subject | Phosphatidylinositol 3-Kinases | en |
dc.subject | Thermogenesis | en |
dc.subject | Transcription Factors | en |
dc.subject | Elsevier B.V. | en |
dc.title | Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes | en |
dc.type | journalArticle | en |