dc.creator | Tsolaki V., Zarogiannis S., Zygoulis P., Kalomenidis I., Jagirdar R., Makris D., Daniil Z., Magkouta S., Triantafyllou I., Papanikolaou J., Gourgoulianis K.I., Zakynthinos E. | en |
dc.date.accessioned | 2023-01-31T10:17:37Z | |
dc.date.available | 2023-01-31T10:17:37Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.1111/resp.13788 | |
dc.identifier.issn | 13237799 | |
dc.identifier.uri | http://hdl.handle.net/11615/80094 | |
dc.description.abstract | Background and objective: Mesothelial cells and cardiomyocytes have shared embryonic mesodermal origin. Cardiomyocytes release BNP under stretch. We searched whether malignant mesothelioma cells also secrete BNP and if so, this has a meaningful impact. Methods: Part I: Prospectively, patients with pleural lesions on CT having malignant mesothelioma effusions (MME, n = 13) were compared to patients with malignant effusions with pleural lesions (MEa, n = 14). Age-matched patients with ME without pleural lesions (MEb, n = 16) and non-malignant effusions (NME, n = 25) were analysed. Part II: Retrospectively, samples from patients with mesothelioma (n = 14), lung cancer (n = 8) or heart failure (n = 9) were used. BNP was measured in pleural fluid and blood/plasma. Part III: BNP was assessed in the culture supernatants of benign (MeT-5A) and malignant mesothelioma cell lines (M14K-epithelioid, MSTO-biphasic and ZL34-sarcomatoid) (n = 10 per cell line in three different biological replicates). Results: In vitro, BNP concentration was significantly higher in the supernatant of all malignant cell lines than benign ones (P < 0.01), denoting BNP's production from the former. The pleural fluid to blood BNP ratio in MME was extremely high in Part I and Part II subjects (28.3 ± 12.1 and 25.9 ± 8.6, respectively) versus 1.1 ± 0.3 and 0.4 ± 0.1 in Part I ME and NME, respectively (P < 0.0001), and 0.8 ± 0.1 and 0.4 ± 0.1 in Part II ME and NME, respectively (P < 0.0001). BNP ratio ≥2.11 in Part I had 92% sensitivity and 94.5% specificity for MME (P < 0.0001). Conclusion: BNP is secreted from malignant mesothelial cells. In clinical practice, the pleural fluid to blood BNP ratio can help in the diagnosis of malignant mesothelioma. © 2020 Asian Pacific Society of Respirology | en |
dc.language.iso | en | en |
dc.source | Respirology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85080975629&doi=10.1111%2fresp.13788&partnerID=40&md5=837fc49326419b73d9d287d1ec883a4b | |
dc.subject | brain natriuretic peptide | en |
dc.subject | fresh frozen plasma | en |
dc.subject | brain natriuretic peptide | en |
dc.subject | aged | en |
dc.subject | Article | en |
dc.subject | blood-to-plasma ratio | en |
dc.subject | cancer cell | en |
dc.subject | cancer diagnosis | en |
dc.subject | cellular secretion | en |
dc.subject | comparative study | en |
dc.subject | computer assisted tomography | en |
dc.subject | controlled study | en |
dc.subject | cytology | en |
dc.subject | diagnostic value | en |
dc.subject | echocardiography | en |
dc.subject | effusion | en |
dc.subject | experimental study | en |
dc.subject | female | en |
dc.subject | Greece | en |
dc.subject | heart failure | en |
dc.subject | histopathology | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | hypervolemia | en |
dc.subject | in vitro study | en |
dc.subject | lung cancer | en |
dc.subject | M14K cell line | en |
dc.subject | major clinical study | en |
dc.subject | male | en |
dc.subject | malignant effusion | en |
dc.subject | malignant mesothelioma effusion | en |
dc.subject | mesothelioma cell line | en |
dc.subject | MeT-5A cell line | en |
dc.subject | MSTO cell line | en |
dc.subject | observational study | en |
dc.subject | pericardial effusion | en |
dc.subject | pleura effusion | en |
dc.subject | pleura fluid | en |
dc.subject | pleura mesothelioma | en |
dc.subject | priority journal | en |
dc.subject | prospective study | en |
dc.subject | protein blood level | en |
dc.subject | protein secretion | en |
dc.subject | reference value | en |
dc.subject | retrospective study | en |
dc.subject | sensitivity and specificity | en |
dc.subject | supernatant | en |
dc.subject | thoracocentesis | en |
dc.subject | thoracoscopy | en |
dc.subject | ZL34 cell line | en |
dc.subject | blood | en |
dc.subject | mesothelioma | en |
dc.subject | metabolism | en |
dc.subject | pathology | en |
dc.subject | tumor cell line | en |
dc.subject | Aged | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Female | en |
dc.subject | Humans | en |
dc.subject | Male | en |
dc.subject | Mesothelioma, Malignant | en |
dc.subject | Natriuretic Peptide, Brain | en |
dc.subject | Prospective Studies | en |
dc.subject | Retrospective Studies | en |
dc.subject | Blackwell Publishing | en |
dc.title | Malignant mesothelioma cells secrete natriuretic peptides: Data and diagnostic clinical implications | en |
dc.type | journalArticle | en |