Histopathological evaluation and redox assessment in blood and kidney tissues in a rabbit contrast-induced nephrotoxicity model
Date
2017Language
en
Keyword
Abstract
Contrast-induced nephropathy (CIN) is a leading cause of hospital-acquired acute kidney injury as a result of iodinated contrast-media use for diagnostic purposes. Pathophysiology remains unclear. In the present study iopromide was administered to New Zealand white rabbits without any prior intervention. Oxidative stress was assessed in blood and tissue level at three anatomical kidney areas (medullary, cortical, juxtamedullary). Histopathological evaluation was also performed. Serum creatinine and urea increased in the CIN groups over 25% at two hours after administration and returned to baseline at 48 h. In kidney tissues, a significant reduction (40%) of catalase in renal cortexes of the CIN groups was observed. Necrosis and tubular vacuolization was also noted that correlated with urea and creatinine levels. Lipid peroxidation decreased at 10 h after administration (>45%) and remained low even at 48 h. Plasma protein carbonyls were significantly increased (67%) in 2 h and dropped later. Serum levels of creatinine and urea at 24 and 48 h significantly correlated with the Total Antioxidant Activity and lipid peroxidation, respectively. Oxidative stress is shown to be involved in CIN development in the rabbit, with more pronounced effects to be confined to the cortex and outer stripe of the outer medulla. © 2017
Collections
Related items
Showing items related by title, author, creator and subject.
-
Clinical effect of accessory renal artery coverage after endovascular repair of aneurysms in abdominal and thoracoabdominal aorta
Spanos K., Nana P., Brotis A.G., Kouvelos G., Behrendt C.-A., Tsilimparis N., Kölbel T., Matsagkas M., Giannoukas A. (2021)Background: The aim of our systematic review and meta-analysis was to assess the effect of accessory renal artery (ARA) coverage on renal function in terms of acute kidney injury (AKI), renal infarction, chronic renal ... -
Renal function impairment in patients undergoing elective EVAR vs. Elective open repair during follow up period: A systematic review of the literature
Spanos K., Karathanos C., Athanasoulas A., Saleptsis V., Vasilopoulos I., Xhepa S., Matsagkas M., Giannoukas A.D. (2017)Background: Endovascular (EVAR) or open surgical (OSR) repair are current treatment options for abdominal aortic aneurysm (AAA). Objective: To produce a systematic review comparing the impact of these 2 treatment options ... -
Age dependence of brachial cuff-based ambulatory PWV in end-stage kidney disease patients undergoing long-term peritoneal dialysis
Vaios V., Georgianos P.I., Vareta G., Divanis D., Dounousi E., Eleftheriadis T., Papagianni A., Zebekakis P.E., Liakopoulos V. (2022)Background: The newly introduced device Mobil-O-Graph (IEM, Stolberg, Germany) combines brachial cuff oscillometry and pulse wave analysis, enabling the determination of pulse wave velocity (PWV) via complex mathematic ...