Εμφάνιση απλής εγγραφής

dc.creatorOsathanunkul M., Madesis P.en
dc.date.accessioned2023-01-31T09:41:11Z
dc.date.available2023-01-31T09:41:11Z
dc.date.issued2021
dc.identifier10.3390/f12111466
dc.identifier.issn19994907
dc.identifier.urihttp://hdl.handle.net/11615/77406
dc.description.abstractThe loss of forests is a major environmental, social, and economic problem. The disappear-ance has been occurring to an extreme degree in many parts of Southeast Asia, including Thailand. Logging and clearing of forests for agriculture, cash crops, and food production has destroyed much of the tropical forests in Thailand. Floristic inventory could provide essential information for forest conservation but species identification as a part of inventory creating could be challenging in some cases. Barcode DNA coupled with High Resolution Melting analysis (Bar-HRM) was used here in aiding species identification of plant in Dipterocarpaceae (Dipterocarpus alatus, D. costatu, D. intricatus, D. obtusifolius, Hopea ferrea, H. odorata, Shorea guiso, S. obtuse, S. roxburghii, and S. siamensis) and Fagaceae (Castanopsis echinocarpa, C. inermis, Lithocarpus wallichianus, Quercus aliena and Q. oidocarpa) families. Two main experiments were conducted including: (1) a comparing method for primer design and (2) testing the robustness of the Bar-HRM by trying to identify tree samples that did not have sequences in the GenBank. In experiment 1, the manual design primer pair was found to be the best fit for the work. Of key importance is finding the primers which give the most nucleotide variations within the generated amplicon; this is a parameter that cannot be set in any web-based tools. Next, in experiment 2, Bar-HRM using primers of ITS1 and ITS2 regions were able to discriminate all 10 tested tree species without any problem, even when there were no sequences of the samples to be analysed before performing the HRM. Here, Bar-HRM poses potential to be a game-changer in tropical forest conservation, as it will be useful for species identification. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.en
dc.language.isoenen
dc.sourceForestsen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85118829151&doi=10.3390%2ff12111466&partnerID=40&md5=7c6e79b478d64c25fc76d0eaa3c86581
dc.subjectBar codesen
dc.subjectCropsen
dc.subjectForestryen
dc.subjectMeltingen
dc.subjectTropicsen
dc.subjectDipterocarpaceaeen
dc.subjectEnvironmental problemsen
dc.subjectFagaceaeen
dc.subjectForest conservationen
dc.subjectHigh resolutionen
dc.subjectHigh resolution melting analyseen
dc.subjectPrimers designen
dc.subjectSpecies identificationen
dc.subjectThailanden
dc.subjectTropical foresten
dc.subjectConservationen
dc.subjectDNAen
dc.subjectforest inventoryen
dc.subjectforest managementen
dc.subjectmeltingen
dc.subjectnature conservationen
dc.subjecttreeen
dc.subjecttropical foresten
dc.subjectFarm Cropsen
dc.subjectForestryen
dc.subjectMeltingen
dc.subjectProduct Codesen
dc.subjectSpecies Identificationen
dc.subjectTropicsen
dc.subjectSoutheast Asiaen
dc.subjectCastanopsis echinocarpaen
dc.subjectDipterocarpaceaeen
dc.subjectDipterocarpus alatusen
dc.subjectFagaceaeen
dc.subjectHopea ferreaen
dc.subjectLithocarpus wallichianusen
dc.subjectQuercus alienaen
dc.subjectShorea guisoen
dc.subjectMDPIen
dc.titleThe identification of several dipterocarpaceae and fagaceae trees by barcode dna coupled with high-resolution melting analysisen
dc.typejournalArticleen


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