dc.creator | Minas I.S., Tanou G., Krokida A., Karagiannis E., Belghazi M., Vasilakakis M., Papadopoulou K.K., Molassiotis A. | en |
dc.date.accessioned | 2023-01-31T09:00:17Z | |
dc.date.available | 2023-01-31T09:00:17Z | |
dc.date.issued | 2018 | |
dc.identifier | 10.1186/s12870-018-1584-y | |
dc.identifier.issn | 14712229 | |
dc.identifier.uri | http://hdl.handle.net/11615/76653 | |
dc.description.abstract | Background: Understanding the mechanisms involved in climacteric fruit ripening is key to improve fruit harvest quality and postharvest performance. Kiwifruit (Actinidia deliciosa cv. 'Hayward') ripening involves a series of metabolic changes regulated by ethylene. Although 1-methylcyclopropene (1-MCP, inhibitor of ethylene action) or ozone (O3) exposure suppresses ethylene-related kiwifruit ripening, how these molecules interact during ripening is unknown. Results: Harvested 'Hayward' kiwifruits were treated with 1-MCP and exposed to ethylene-free cold storage (0 °C, RH 95%) with ambient atmosphere (control) or atmosphere enriched with O3 (0.3 μL L- 1) for up to 6 months. Their subsequent ripening performance at 20 °C (90% RH) was characterized. Treatment with either 1-MCP or O3 inhibited endogenous ethylene biosynthesis and delayed fruit ripening at 20 °C. 1-MCP and O3 in combination severely inhibited kiwifruit ripening, significantly extending fruit storage potential. To characterize ethylene sensitivity of kiwifruit following 1-MCP and O3 treatments, fruit were exposed to exogenous ethylene (100 μL L- 1, 24 h) upon transfer to 20 °C following 4 and 6 months of cold storage. Exogenous ethylene treatment restored ethylene biosynthesis in fruit previously exposed in an O3-enriched atmosphere. Comparative proteomics analysis showed separate kiwifruit ripening responses, unraveled common 1-MCP- and O3-dependent metabolic pathways and identified specific proteins associated with these different ripening behaviors. Protein components that were differentially expressed following exogenous ethylene exposure after 1-MCP or O3 treatment were identified and their protein-protein interaction networks were determined. The expression of several kiwifruit ripening related genes, such as 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1), ethylene receptor (ETR1), lipoxygenase (LOX1), geranylgeranyl diphosphate synthase (GGP1), and expansin (EXP2), was strongly affected by O3, 1-MCP, their combination, and exogenously applied ethylene. Conclusions: Our findings suggest that the combination of 1-MCP and O3 functions as a robust repressive modulator of kiwifruit ripening and provide new insight into the metabolic events underlying ethylene-induced and ethylene-independent ripening outcomes. © 2018 The Author(s). | en |
dc.language.iso | en | en |
dc.source | BMC Plant Biology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058663591&doi=10.1186%2fs12870-018-1584-y&partnerID=40&md5=1b344f810440075b30a6ceec10318b5c | |
dc.subject | 1-methylcyclopropene | en |
dc.subject | cyclopropane derivative | en |
dc.subject | ethylene | en |
dc.subject | ethylene derivative | en |
dc.subject | ozone | en |
dc.subject | plant protein | en |
dc.subject | transcription factor | en |
dc.subject | Actinidia | en |
dc.subject | drug effect | en |
dc.subject | food storage | en |
dc.subject | fruit | en |
dc.subject | gene expression regulation | en |
dc.subject | genetics | en |
dc.subject | metabolism | en |
dc.subject | physiology | en |
dc.subject | signal transduction | en |
dc.subject | Actinidia | en |
dc.subject | Cyclopropanes | en |
dc.subject | Ethylenes | en |
dc.subject | Food Storage | en |
dc.subject | Fruit | en |
dc.subject | Gene Expression Regulation, Plant | en |
dc.subject | Ozone | en |
dc.subject | Plant Proteins | en |
dc.subject | Signal Transduction | en |
dc.subject | Transcription Factors | en |
dc.subject | BioMed Central Ltd. | en |
dc.title | Ozone-induced inhibition of kiwifruit ripening is amplified by 1-methylcyclopropene and reversed by exogenous ethylene | en |
dc.type | journalArticle | en |