dc.creator | Kerasioti E., Stagos D., Tzimi A., Kouretas D. | en |
dc.date.accessioned | 2023-01-31T08:43:14Z | |
dc.date.available | 2023-01-31T08:43:14Z | |
dc.date.issued | 2016 | |
dc.identifier | 10.1016/j.fct.2016.08.022 | |
dc.identifier.issn | 02786915 | |
dc.identifier.uri | http://hdl.handle.net/11615/74861 | |
dc.description.abstract | The aim of the present study was to investigate the molecular mechanisms through which sheep/goat whey protein exerts its antioxidant activity. Thus, it was examined whey protein's effects on the expression of transcription factor, nuclear factor-like 2 (Nrf2) and on the expression and activity of a number of antioxidant and phase II enzymes, superoxide dismutase (SOD), catalase (CAT), heme oxygenase 1 (HO-1), synthase glutamyl cysteine (GCS) and glutathione-s-transferase (GST), in muscle C2C12 and EA.hy926 endothelial cells. C2C12 and EA.hy926 cells were treated with sheep/goat whey protein (0.78 and 3.12 mg/ml) and incubated for 3, 6, 12, 18 and 24 h. Whey protein increased significantly the expression of Nrf2 only in EA.hy926 cells. Also, the expression of SOD, HO-1, CAT and the activity of SOD, CAT and GST were increased significantly in both cells types. The expression of GCS was increased significantly only in C2C12 cells. Sheep/goat whey protein was shown for the first time to exert its antioxidant activity through Nrf2-dependent mechanism in endothelial cells and Nrf2-independent mechanism in muscle cells. Thus, Nrf2 could be a target for food supplements containing whey protein in order to prevent oxidative stress damages and diseases related to endothelium. © 2016 Elsevier Ltd | en |
dc.language.iso | en | en |
dc.source | Food and Chemical Toxicology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989339537&doi=10.1016%2fj.fct.2016.08.022&partnerID=40&md5=9ef7bc8375f2d1a79ada46486b9e930d | |
dc.subject | catalase | en |
dc.subject | cysteine synthase | en |
dc.subject | glutamyl cysteine synthase | en |
dc.subject | glutathione transferase | en |
dc.subject | heme oxygenase 1 | en |
dc.subject | superoxide dismutase | en |
dc.subject | transcription factor Nrf2 | en |
dc.subject | unclassified drug | en |
dc.subject | whey protein | en |
dc.subject | antioxidant | en |
dc.subject | transcription factor Nrf2 | en |
dc.subject | whey protein | en |
dc.subject | antioxidant activity | en |
dc.subject | Article | en |
dc.subject | cell specificity | en |
dc.subject | controlled study | en |
dc.subject | drug mechanism | en |
dc.subject | endothelium cell | en |
dc.subject | enzyme activity | en |
dc.subject | goat | en |
dc.subject | muscle cell | en |
dc.subject | nonhuman | en |
dc.subject | protein expression | en |
dc.subject | sheep | en |
dc.subject | Western blotting | en |
dc.subject | animal | en |
dc.subject | cell culture | en |
dc.subject | drug effects | en |
dc.subject | gene expression regulation | en |
dc.subject | genetics | en |
dc.subject | metabolism | en |
dc.subject | myoblast | en |
dc.subject | oxidative stress | en |
dc.subject | vascular endothelium | en |
dc.subject | Animals | en |
dc.subject | Antioxidants | en |
dc.subject | Cells, Cultured | en |
dc.subject | Endothelium, Vascular | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | Goats | en |
dc.subject | Myoblasts | en |
dc.subject | NF-E2-Related Factor 2 | en |
dc.subject | Oxidative Stress | en |
dc.subject | Sheep | en |
dc.subject | Whey Proteins | en |
dc.subject | Elsevier Ltd | en |
dc.title | Increase in antioxidant activity by sheep/goat whey protein through nuclear factor-like 2 (Nrf2) is cell type dependent | en |
dc.type | journalArticle | en |