dc.creator | Katsafadou A.I., Vasileiou N.G.C., Tsangaris G.T., Ioannidi K.S., Anagnostopoulos A.K., Billinis C., Fragkou I.A., Papadopoulos E., Mavrogianni V.S., Lianou D.T., Chatzopoulos D.C., Fthenakis G.C. | en |
dc.date.accessioned | 2023-01-31T08:33:21Z | |
dc.date.available | 2023-01-31T08:33:21Z | |
dc.date.issued | 2021 | |
dc.identifier | 10.2174/1570164617999200510234638 | |
dc.identifier.issn | 15701646 | |
dc.identifier.uri | http://hdl.handle.net/11615/74604 | |
dc.description.abstract | Aims: The importance of cathelicidin-1 as an indicator of the severity of mammary infection in ewes. Background. Mastitis is an important disease of sheep, affecting their health and welfare. Objective: The association of the presence of cathelicidin-1 in milk samples from ewes with masti-tis with the severity of the infection. Methods: Ewes were intramammarily inoculated with Mannheimia haemolytica or Staphylococcus chromogenes. Conventional (clinical, bacteriological and cytological examinations; milk yield mea-surements) and proteomics evaluation (2-DE, MALDI-TOF MS) to record cathelicidin-1 spot opti-cal densities in milk samples were recorded. Results: Ewes challenged with M. haemolytica developed clinical and ewes challenged with S. chromogenes subclinical mastitis (P=0.05). The challenged organism was isolated from milk samples from inoculated mammary glands; increased somatic cell counts were also recorded. Catheli-cidin-1 was detected in milk samples from the inoculated side of udders of all ewes. Mean spot density of cathelicidin-1 from samples from inoculated glands of ewes challenged with M. haemolytica was higher than from ewes challenged with S. chromogenes: 2896 ± 973 versus 1312 ± 361 (P =0.034). There were significant correlations between the presence of clinical mastitis / somatic cell counts with the spot density of cathelicidin-1 on 2-DE gels (P=0.043 and P=0.023, respectively). There was also a significant inverse correlation between the mean spot densities of cathelicidin-1 in milk samples and the milk yield of respective ewes on D10 (P =0.031). Conclusion: Potentially, cathelicidin-1 could be used as a marker to indicate the severity of damage to the mammary parenchyma. © 2021 Bentham Science Publishers. | en |
dc.language.iso | en | en |
dc.source | Current Proteomics | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101247642&doi=10.2174%2f1570164617999200510234638&partnerID=40&md5=3cb43660ff3a27526f89518b45fbc2c1 | |
dc.subject | cathelicidin | en |
dc.subject | cathelicidin 1 | en |
dc.subject | unclassified drug | en |
dc.subject | animal cell | en |
dc.subject | animal experiment | en |
dc.subject | Article | en |
dc.subject | bacterium examination | en |
dc.subject | breast epithelium cell | en |
dc.subject | cell count | en |
dc.subject | clinical examination | en |
dc.subject | controlled study | en |
dc.subject | cytology | en |
dc.subject | disease severity | en |
dc.subject | ewe | en |
dc.subject | female | en |
dc.subject | image analysis | en |
dc.subject | mammary gland | en |
dc.subject | Mannheimia haemolytica | en |
dc.subject | mastitis | en |
dc.subject | matrix assisted laser desorption ionization time of flight mass spectrometry | en |
dc.subject | milk | en |
dc.subject | milk yield | en |
dc.subject | nonhuman | en |
dc.subject | optical density | en |
dc.subject | pathogenesis | en |
dc.subject | peptide mass fingerprinting | en |
dc.subject | priority journal | en |
dc.subject | protein analysis | en |
dc.subject | protein expression | en |
dc.subject | proteomics | en |
dc.subject | sheep disease | en |
dc.subject | somatic cell | en |
dc.subject | Staphylococcus | en |
dc.subject | udder | en |
dc.subject | Bentham Science Publishers | en |
dc.title | Presence of cathelicidin-1 in milk as an indicator of the severity of mammary infection in ewes | en |
dc.type | journalArticle | en |