Εμφάνιση απλής εγγραφής

dc.creatorHemati Z., Meletis E., Derakhshandeh A., Haghkhah M., Kostoulas P., Singh S.V., Chaubey K.K., Gupta S.en
dc.date.accessioned2023-01-31T08:28:05Z
dc.date.available2023-01-31T08:28:05Z
dc.date.issued2022
dc.identifier10.1186/s12917-022-03141-7
dc.identifier.issn17466148
dc.identifier.urihttp://hdl.handle.net/11615/73947
dc.description.abstractBackground: This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the detection of antibodies against MAP. Four diagnostic applied tests were evaluated including Indigenous plate-ELISA (IP-ELISA), Mt-ELISA, fecal Polymerase Chain Reaction (f-PCR) and fecal culture (FC). Materials and methods: Assuming the absence of a gold standard, latent-class models in a Bayesian framework were used to estimate the diagnostic accuracy of the four tests for MAP. Results: Mt-ELISA had higher Sensitivity (Se) in sheep (posterior median: 0.68 (95% Probability Interval (PI): 0.43–0.95), while IP-ELISA recorded the highest Se in goats as 0.83 (95% PI, 0.61–0.97). The f-PCR Se estimate slightly differed between species [sheep 0.36 (0.19–0.58), goats 0.19 (0.08–0.35)], while the Se of FC was similar between species [sheep 0.29 (0.15–0.51), goats 0.27 (0.13–0.45)]. The specificity estimates for all tests were high, close to unity, and similar between species. Conclusion: Overall, the results showed that the Mt-ELISA method can be used for MAP detection in small ruminants’ flocks. © 2022, The Author(s).en
dc.language.isoenen
dc.sourceBMC Veterinary Researchen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85123089183&doi=10.1186%2fs12917-022-03141-7&partnerID=40&md5=f207932b484dd6518df84199064c1f89
dc.subjectbacterium antibodyen
dc.subjectanimal experimenten
dc.subjectanimal modelen
dc.subjectArticleen
dc.subjectbacterium cultureen
dc.subjectcommunity structureen
dc.subjectcontrolled studyen
dc.subjectdairy cattleen
dc.subjectdiagnostic accuracyen
dc.subjectdiagnostic test accuracy studyen
dc.subjectenzyme linked immunosorbent assayen
dc.subjectfeces analysisen
dc.subjectgoaten
dc.subjectimmune responseen
dc.subjectMycobacterium avium subsp. paratuberculosisen
dc.subjectnonhumanen
dc.subjectparatuberculosisen
dc.subjectplate ELISAen
dc.subjectpolyacrylamide gel electrophoresisen
dc.subjectpolymerase chain reactionen
dc.subjectprevalenceen
dc.subjectruminanten
dc.subjectsheepen
dc.subjectanimalen
dc.subjectBayes theoremen
dc.subjectfecesen
dc.subjectgoat diseaseen
dc.subjectMycobacterium avium subsp. paratuberculosisen
dc.subjectsensitivity and specificityen
dc.subjectsheep diseaseen
dc.subjectveterinary medicineen
dc.subjectAnimalsen
dc.subjectBayes Theoremen
dc.subjectEnzyme-Linked Immunosorbent Assayen
dc.subjectFecesen
dc.subjectGoat Diseasesen
dc.subjectGoatsen
dc.subjectMycobacterium avium subsp. paratuberculosisen
dc.subjectParatuberculosisen
dc.subjectSensitivity and Specificityen
dc.subjectSheepen
dc.subjectSheep Diseasesen
dc.subjectBioMed Central Ltden
dc.titleApplication of Bayesian modeling for diagnostic assays of Mycobacterium avium subsp. paratuberculosis in sheep and goats flocksen
dc.typejournalArticleen


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