| dc.creator | Garagounis C., Georgopoulou M.-E., Beritza K., Papadopoulou K.K. | en |
| dc.date.accessioned | 2023-01-31T07:39:37Z | |
| dc.date.available | 2023-01-31T07:39:37Z | |
| dc.date.issued | 2020 | |
| dc.identifier | 10.1016/j.mex.2020.101098 | |
| dc.identifier.issn | 22150161 | |
| dc.identifier.uri | http://hdl.handle.net/11615/71941 | |
| dc.description.abstract | • This work describes a protocol for hairy root transformation of the medicinal crop legume fenugreek (Trigonella foenum-graecum L.). Hairy root plant transformation mediated by Agrobacterium rhizogenes is an established method for the rapid genetic transformation of various dicotyledonous plants. We have adapted a hairy root transformation protocol from the model legume Medicago truncatula for use in this metabolically rich non-model crop legume. Considering the great variety and abundance of phytochemicals in fenugreek and its established use in traditional medicine, we aim for this method to become a resource for metabolic pathway identification and for production of valuable specialised metabolites via metabolic engineering approaches. • Development rapid transformation (2.5–3 weeks) of fenugreek roots via A. rhizogenes. • Marker gene cassette with suitable promoter for visual detection of transformed fenugreek roots © 2020 | en |
| dc.language.iso | en | en |
| dc.source | MethodsX | en |
| dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092499540&doi=10.1016%2fj.mex.2020.101098&partnerID=40&md5=18c5a355202f9c3623e670fe7b75db24 | |
| dc.subject | Article | en |
| dc.subject | bacterial strain | en |
| dc.subject | barrel medic | en |
| dc.subject | coculture | en |
| dc.subject | controlled study | en |
| dc.subject | fenugreek | en |
| dc.subject | gene cassette | en |
| dc.subject | germination rate | en |
| dc.subject | hairy root culture | en |
| dc.subject | hypocotyl | en |
| dc.subject | marker gene | en |
| dc.subject | metabolic engineering | en |
| dc.subject | nonhuman | en |
| dc.subject | nutrient solution | en |
| dc.subject | plant growth | en |
| dc.subject | plant root | en |
| dc.subject | plant seed | en |
| dc.subject | priority journal | en |
| dc.subject | Rhizobium rhizogenes | en |
| dc.subject | seedling | en |
| dc.subject | shoot | en |
| dc.subject | tissue culture | en |
| dc.subject | Elsevier B.V. | en |
| dc.title | An Agrobacterium rhizogenes mediated hairy root transformation protocol for fenugreek | en |
| dc.type | journalArticle | en |
