Phytochemical, antioxidant, enzyme activity and antifungal properties of Satureja khuzistanica in vitro and in vivo explants stimulated by some chemical elicitors
Data
2020Language
en
Soggetto
Abstract
Context:Satureja khuzistanica Jamzad. (Lamiaceae), is known for its antifungal and antioxidant compounds, especially rosmarinic acid (RA). Objective: The study examines the effect of elicitors on RA production and phytochemical properties of S. khuzistanica. Materials and methods:In vitro plants were treated with methyl jasmonate (MeJA) and multi-walled carbon nanotubes (MWCNTs). In vivo plants were treated with MWCNTs and salicylic acid (SA). RA was measured by HPLC. Catalase (CAT), guaiacol peroxidase (POD) and ascorbate peroxidase (APX) were quantified. DPPH and β-carotene were assayed in in vivo extracts. The antifungal effects of extracts were evaluated against Fusarium solani K (FsK). Results: The highest RA contents of in vitro plants were 50 mg/L MeJA (140.99 mg/g DW) and 250 mg/L MWCNTs (140.49 mg/g DW). The highest in vivo were 24 h MWCNTs (7.13 mg/g DW) and 72 h SA (9.12 mg/g DW). The maximum POD and APX activities were at 100 mg/L MeJA (5 and 4 mg protein, respectively). CAT had the highest activities at 50 mg/L MeJA (2 mg protein). DPPH and β-carotene showed 50% and 80% inhibition, respectively. The FsK aggregation was the lowest for in vitro extract in number of conidia [1.82 × 1010], fresh weight (6.51 g) and dry weight (0.21 g) that proved RA inhibitory effects. The callus reduces FsK growth diameter to 2.75 on the 5th day. Discussion and conclusions: Application of MeJA, SA, and MWCNTSs could increase RA in S. khuzistanica and highlighted potential characteristics in pharmaceutical and antifungal effects. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Extracts from the mediterranean food plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum enhanced GSH levels and increased Nrf2 expression in human endothelial cells
Stagos D., Balabanos D., Savva S., Skaperda Z., Priftis A., Kerasioti E., Mikropoulou E.V., Vougogiannopoulou K., Mitakou S., Halabalaki M., Kouretas D. (2018)The Mediterranean diet is considered to prevent several diseases. In the present study, the antioxidant properties of six extracts from Mediterranean plant foods were assessed. The extracts' chemical composition analysis ... -
The combined and single effect of salinity and copper stress on growth and quality of Mentha spicata plants
Chrysargyris A., Papakyriakou E., Petropoulos S.A., Tzortzakis N. (2019)Copper is essential for plant growth, but in excess may cause adverse effects on plant physiology. Harmful effects are also caused by plant exposure to salinity (NaCl) due to the excessive use of fertilizers, soil degradation ... -
Chemical composition and antioxidant activity of Cichorium spinosum L. leaves in relation to developmental stage
Petropoulos S.A., Fernandes Â., Antoniadis V., Ntatsi G., Barros L., Ferreira I.C.F.R. (2018)In the present study, chemical composition and bioactivity of Cichorium spinosum leaves were examined at different growth stages for optimum harvest stage and end-use assessment. Total fresh weight and number of leaves ...