dc.creator | Eleftheriadis T., Pissas G., Crespo M., Nikolaou E., Liakopoulos V., Stefanidis I. | en |
dc.date.accessioned | 2023-01-31T07:37:15Z | |
dc.date.available | 2023-01-31T07:37:15Z | |
dc.date.issued | 2021 | |
dc.identifier | 10.3390/ijms22041733 | |
dc.identifier.issn | 16616596 | |
dc.identifier.uri | http://hdl.handle.net/11615/71330 | |
dc.description.abstract | Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | en |
dc.language.iso | en | en |
dc.source | International Journal of Molecular Sciences | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100536536&doi=10.3390%2fijms22041733&partnerID=40&md5=952b049d3548431d07936e4629ca38fd | |
dc.subject | alloantigen | en |
dc.subject | adverse event | en |
dc.subject | allograft | en |
dc.subject | allotransplantation | en |
dc.subject | antigen presentation | en |
dc.subject | CD4+ T lymphocyte | en |
dc.subject | cell culture | en |
dc.subject | coculture | en |
dc.subject | cytology | en |
dc.subject | epithelium cell | en |
dc.subject | graft rejection | en |
dc.subject | human | en |
dc.subject | immunology | en |
dc.subject | kidney proximal tubule | en |
dc.subject | kidney transplantation | en |
dc.subject | lymphocyte activation | en |
dc.subject | pathology | en |
dc.subject | primary cell culture | en |
dc.subject | reperfusion injury | en |
dc.subject | Allografts | en |
dc.subject | Antigen Presentation | en |
dc.subject | CD4-Positive T-Lymphocytes | en |
dc.subject | Cells, Cultured | en |
dc.subject | Coculture Techniques | en |
dc.subject | Epithelial Cells | en |
dc.subject | Graft Rejection | en |
dc.subject | Humans | en |
dc.subject | Isoantigens | en |
dc.subject | Kidney Transplantation | en |
dc.subject | Kidney Tubules, Proximal | en |
dc.subject | Lymphocyte Activation | en |
dc.subject | Primary Cell Culture | en |
dc.subject | Reperfusion Injury | en |
dc.subject | Transplantation, Homologous | en |
dc.subject | MDPI AG | en |
dc.title | A role for human renal tubular epithelial cells in direct allo-recognition by CD4+ T-cells and the effect of ischemia-reperfusion | en |
dc.type | journalArticle | en |