dc.creator | Eleftheriadis T., Pissas G., Crespo M., Filippidis G., Antoniadis N., Liakopoulos V., Stefanidis I. | en |
dc.date.accessioned | 2023-01-31T07:37:15Z | |
dc.date.available | 2023-01-31T07:37:15Z | |
dc.date.issued | 2021 | |
dc.identifier | 10.3892/mmr.2021.11994 | |
dc.identifier.issn | 17912997 | |
dc.identifier.uri | http://hdl.handle.net/11615/71328 | |
dc.description.abstract | In antibody‑mediated rejection (ABMR), the graft endothelium is at the forefront of the kidney transplant against the assault from the recipient's humoral immune system, and is a target of the latter. The present study investigated the effect of antibodies against human leukocyte antigen (HLA) class I (anti‑HLAI) on the immunological properties of human glomerular endothelial cells. Additionally, the effect of the mammalian target of rapamycin (mTOR) complex 1 (mTORC1) inhibitor (everolimus), or the general control nonderepress‑ ible 2 kinase (GCN2K) activator (halofuginone) on anti‑HLAI antibody‑mediated alterations was assessed. Cell integrity was examined, an lactate dehydrogenase (LDH) release assay was performed and cleaved caspase‑3 levels were determined. Furthermore, cell proliferation was analyzed by performing a bromodeoxyuridine assay and the cellular proteins involved in signal transduction or immune effector mechanisms were assessed via western blotting. IL‑8, monocyte chemoattrac‑ tive protein‑1 (MCP‑1), von Willebrand factor (vWF) and transforming growth factor‑beta 1 (TGF‑β1) were assayed via ELISA. The results revealed that anti‑HLAI triggered integrin signaling, activated mTOR and GCN2K, preserved cell integrity and promoted cell proliferation. Additionally, by increasing intercellular adhesion molecule 1 (ICAM‑1), HLA‑DR, IL‑8 and MCP‑1 levels, anti‑HLAI enhanced the ability of immune cells to interact with endothelial cells thus facilitating graft rejection. Contrarily, by upregulating CD46 and CD59, anti‑HLAI rendered the endothelium less vulner‑ able to complement‑mediated injury. Finally, by enhancing vWF and TGF‑β1, anti‑HLAI may render the endothelium prothrombotic and facilitate fibrosis and graft failure, respec‑ tively. According to our results, mTORC1 inhibition and GCN2K activation may prove useful pharmaceutical targets, as they prevent cell proliferation and downregulate ICAM‑1, IL‑8, MCP‑1 and TGF‑β1. mTORC1 inhibition also decreases vWF. © 2021 Spandidos Publications. All rights reserved. | en |
dc.language.iso | en | en |
dc.source | Molecular Medicine Reports | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102523078&doi=10.3892%2fmmr.2021.11994&partnerID=40&md5=74932c1c800f4ee7544122dd18520633 | |
dc.subject | antiidiotypic antibody | en |
dc.subject | CD59 antigen | en |
dc.subject | EIF2AK4 protein, human | en |
dc.subject | everolimus | en |
dc.subject | HLA antigen class 1 | en |
dc.subject | mammalian target of rapamycin complex 1 | en |
dc.subject | membrane cofactor protein | en |
dc.subject | MTOR protein, human | en |
dc.subject | protein serine threonine kinase | en |
dc.subject | target of rapamycin kinase | en |
dc.subject | von Willebrand factor | en |
dc.subject | adverse event | en |
dc.subject | cell proliferation | en |
dc.subject | drug effect | en |
dc.subject | endothelium cell | en |
dc.subject | genetics | en |
dc.subject | graft rejection | en |
dc.subject | human | en |
dc.subject | humoral immunity | en |
dc.subject | immunology | en |
dc.subject | kidney transplantation | en |
dc.subject | pathology | en |
dc.subject | primary cell culture | en |
dc.subject | signal transduction | en |
dc.subject | Antibodies, Anti-Idiotypic | en |
dc.subject | CD59 Antigens | en |
dc.subject | Cell Proliferation | en |
dc.subject | Endothelial Cells | en |
dc.subject | Everolimus | en |
dc.subject | Graft Rejection | en |
dc.subject | Histocompatibility Antigens Class I | en |
dc.subject | Humans | en |
dc.subject | Immunity, Humoral | en |
dc.subject | Kidney Transplantation | en |
dc.subject | Mechanistic Target of Rapamycin Complex 1 | en |
dc.subject | Membrane Cofactor Protein | en |
dc.subject | Primary Cell Culture | en |
dc.subject | Protein-Serine-Threonine Kinases | en |
dc.subject | Signal Transduction | en |
dc.subject | TOR Serine-Threonine Kinases | en |
dc.subject | von Willebrand Factor | en |
dc.subject | Spandidos Publications | en |
dc.title | The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation | en |
dc.type | journalArticle | en |