dc.creator | Befani C., Liakos P. | en |
dc.date.accessioned | 2023-01-31T07:37:09Z | |
dc.date.available | 2023-01-31T07:37:09Z | |
dc.date.issued | 2017 | |
dc.identifier | 10.1002/cbin.10777 | |
dc.identifier.issn | 10656995 | |
dc.identifier.uri | http://hdl.handle.net/11615/71298 | |
dc.description.abstract | Tissue hypoxia affects gene expression through the hypoxia-inducible transcription factors, HIF-1 and HIF-2, in both physiological and pathological angiogenesis. Angiogenesis is a complex response of endothelial cells integrating cell proliferation, migration, tube formation, and their interaction with the extracellular matrix through integrin receptors. In this report, we studied the effect of hypoxia on the angiogenic functions of human microvascular endothelial cells (HMEC-1) as well as on expression of the angiogenic integrins ανβ3, ανβ5, and α5β1. Exposure of HMEC-1 to hypoxia (1% O2) or to DMOG, a prolyl-4-hydroxylase inhibitor, caused significant reduction to their proliferation rate, whereas their migration ability toward laminin-1 or collagen IV and capillary-like tube formation were significantly enhanced. In addition, αv, β1, β3, and β5 integrins expression was increased under hypoxia in HMEC-1, while α5 integrin was not affected. Both HIF-1 and HIF-2 protein expression and transcriptional activity were induced under hypoxia in HMEC-1. The knockdown of either HIF-1α or HIF-2α inhibited integrin β3 hypoxic stimulation, suggesting a HIF-dependent induction of β3 integrin in HMEC-1. Taken together, our results indicate that hypoxia transcriptionally up-regulates angiogenic integrins in microvascular endothelial cells along with promoting migration and tube formation of HMEC-1. © 2017 International Federation for Cell Biology | en |
dc.language.iso | en | en |
dc.source | Cell Biology International | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018761369&doi=10.1002%2fcbin.10777&partnerID=40&md5=68918c7bb8b4aca31c70eb118aee139c | |
dc.subject | alphaVbeta1 integrin | en |
dc.subject | alphaVbeta5 integrin | en |
dc.subject | beta1 integrin | en |
dc.subject | beta3 integrin | en |
dc.subject | beta5 integrin | en |
dc.subject | CD51 antigen | en |
dc.subject | collagen type 4 | en |
dc.subject | hypoxia inducible factor 1 | en |
dc.subject | hypoxia inducible factor 1alpha | en |
dc.subject | hypoxia inducible factor 2 | en |
dc.subject | hypoxia inducible factor 2alpha | en |
dc.subject | integrin | en |
dc.subject | laminin 1 | en |
dc.subject | prolyl hydroxylase inhibitor | en |
dc.subject | RNA | en |
dc.subject | small interfering RNA | en |
dc.subject | unclassified drug | en |
dc.subject | vitronectin receptor | en |
dc.subject | basic helix loop helix transcription factor | en |
dc.subject | endothelial PAS domain-containing protein 1 | en |
dc.subject | HIF1A protein, human | en |
dc.subject | hypoxia inducible factor 1alpha | en |
dc.subject | integrin | en |
dc.subject | messenger RNA | en |
dc.subject | Article | en |
dc.subject | capillary endothelial cell | en |
dc.subject | cell migration | en |
dc.subject | cell proliferation | en |
dc.subject | controlled study | en |
dc.subject | exposure | en |
dc.subject | gene expression | en |
dc.subject | genetic transfection | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | human cell culture | en |
dc.subject | hypoxia | en |
dc.subject | protein expression | en |
dc.subject | protein induction | en |
dc.subject | real time polymerase chain reaction | en |
dc.subject | RNA extraction | en |
dc.subject | upregulation | en |
dc.subject | Western blotting | en |
dc.subject | angiogenesis | en |
dc.subject | biosynthesis | en |
dc.subject | cell hypoxia | en |
dc.subject | cell motion | en |
dc.subject | cytology | en |
dc.subject | endothelium cell | en |
dc.subject | genetics | en |
dc.subject | hypoxia | en |
dc.subject | metabolism | en |
dc.subject | pathophysiology | en |
dc.subject | physiology | en |
dc.subject | vascular endothelium | en |
dc.subject | Basic Helix-Loop-Helix Transcription Factors | en |
dc.subject | Cell Hypoxia | en |
dc.subject | Cell Movement | en |
dc.subject | Cell Proliferation | en |
dc.subject | Endothelial Cells | en |
dc.subject | Endothelium, Vascular | en |
dc.subject | Humans | en |
dc.subject | Hypoxia | en |
dc.subject | Hypoxia-Inducible Factor 1, alpha Subunit | en |
dc.subject | Integrins | en |
dc.subject | Neovascularization, Physiologic | en |
dc.subject | RNA, Messenger | en |
dc.subject | Up-Regulation | en |
dc.subject | Wiley-Blackwell Publishing Ltd | en |
dc.title | Hypoxia upregulates integrin gene expression in microvascular endothelial cells and promotes their migration and capillary-like tube formation | en |
dc.type | journalArticle | en |