dc.creator | Bartosova M., Herzog R., Ridinger D., Levai E., Jenei H., Zhang C., González Mateo G.T., Marinovic I., Hackert T., Bestvater F., Hausmann M., Cabrera M.L., Kratochwill K., Zarogiannis S.G., Schmitt C.P. | en |
dc.date.accessioned | 2023-01-31T07:36:11Z | |
dc.date.available | 2023-01-31T07:36:11Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.3390/biom10081178 | |
dc.identifier.issn | 2218273X | |
dc.identifier.uri | http://hdl.handle.net/11615/71139 | |
dc.description.abstract | Understanding and targeting the molecular basis of peritoneal solute and protein transport is essential to improve peritoneal dialysis (PD) efficacy and patient outcome. Supplementation of PD fluids (PDF) with alanyl-glutamine (AlaGln) increased small solute transport and reduced peritoneal protein loss in a recent clinical trial. Transepithelial resistance and 10 kDa and 70 kDa dextran transport were measured in primary human endothelial cells (HUVEC) exposed to conventional acidic, glucose degradation products (GDP) containing PDF (CPDF) and to low GDP containing PDF (LPDF) with and without AlaGln. Zonula occludens-1 (ZO-1) and claudin-5 were quantified by Western blot and immunofluorescence and in mice exposed to saline and CPDF for 7 weeks by digital imaging analyses. Spatial clustering of ZO-1 molecules was assessed by single molecule localization microscopy. AlaGln increased transepithelial resistance, and in CPDF exposed HUVEC decreased dextran transport rates and preserved claudin-5 and ZO-1 abundance. Endothelial clustering of membrane bound ZO-1 was higher in CPDF supplemented with AlaGln. In mice, arteriolar endothelial claudin-5 was reduced in CPDF, but restored with AlaGln, while mesothelial claudin-5 abundance was unchanged. AlaGln supplementation seals the peritoneal endothelial barrier, and when supplemented to conventional PD fluid increases claudin-5 and ZO-1 abundance and clustering of ZO-1 in the endothelial cell membrane. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | en |
dc.language.iso | en | en |
dc.source | Biomolecules | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089567415&doi=10.3390%2fbiom10081178&partnerID=40&md5=e2f439d1dd424e958015b727b010ed30 | |
dc.subject | alanyl glutamine | en |
dc.subject | avidin | en |
dc.subject | balance | en |
dc.subject | biotin | en |
dc.subject | calcium | en |
dc.subject | dextran | en |
dc.subject | diaminobenzidine | en |
dc.subject | fluorescein isothiocyanate | en |
dc.subject | glucose | en |
dc.subject | glutamine | en |
dc.subject | glyceraldehyde 3 phosphate dehydrogenase | en |
dc.subject | hematoxylin | en |
dc.subject | lactate dehydrogenase | en |
dc.subject | mercaptoethanol | en |
dc.subject | peritoneal dialysis fluid | en |
dc.subject | peroxidase | en |
dc.subject | platelet endothelial cell adhesion molecule 1 | en |
dc.subject | polyacrylamide gel | en |
dc.subject | rabbit antiserum | en |
dc.subject | staysafe | en |
dc.subject | unclassified drug | en |
dc.subject | alanylglutamine | en |
dc.subject | claudin 5 | en |
dc.subject | CLDN5 protein, human | en |
dc.subject | dialysis fluid | en |
dc.subject | dipeptide | en |
dc.subject | protein ZO1 | en |
dc.subject | TJP1 protein, human | en |
dc.subject | animal cell | en |
dc.subject | animal model | en |
dc.subject | animal tissue | en |
dc.subject | Article | en |
dc.subject | C57BL 6 mouse | en |
dc.subject | cytotoxicity | en |
dc.subject | densitometry | en |
dc.subject | endothelium cell | en |
dc.subject | female | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | HUVEC cell line | en |
dc.subject | immunofluorescence test | en |
dc.subject | immunohistochemistry | en |
dc.subject | mouse | en |
dc.subject | nonhuman | en |
dc.subject | paraffin embedding | en |
dc.subject | tight junction | en |
dc.subject | transendothelial electrical resistance | en |
dc.subject | Western blotting | en |
dc.subject | adverse event | en |
dc.subject | animal | en |
dc.subject | disease model | en |
dc.subject | drug effect | en |
dc.subject | metabolism | en |
dc.subject | peritoneal dialysis | en |
dc.subject | single molecule imaging | en |
dc.subject | tight junction | en |
dc.subject | transport at the cellular level | en |
dc.subject | umbilical vein endothelial cell | en |
dc.subject | Animals | en |
dc.subject | Biological Transport | en |
dc.subject | Claudin-5 | en |
dc.subject | Dialysis Solutions | en |
dc.subject | Dipeptides | en |
dc.subject | Disease Models, Animal | en |
dc.subject | Female | en |
dc.subject | Human Umbilical Vein Endothelial Cells | en |
dc.subject | Humans | en |
dc.subject | Mice | en |
dc.subject | Peritoneal Dialysis | en |
dc.subject | Single Molecule Imaging | en |
dc.subject | Tight Junctions | en |
dc.subject | Zonula Occludens-1 Protein | en |
dc.subject | MDPI AG | en |
dc.title | Alanyl-glutamine restores tight junction organization after disruption by a conventional peritoneal dialysis fluid | en |
dc.type | journalArticle | en |