Post mortem aqueous humor analysis in sheep as index of ante mortem serum biochemistry profile

Abstract

We investigated the usefulness of the concentration of total proteins, albumin, creatinine, calcium (Ca), magnesium (Mg) and the activities of aspartate aminotransferase (AST) and gamma-glutamyltransferase (GGT) in aqueous humor in estimating the ante mortem levels of the same biochemical analytes in sheep blood. A blood sample was taken ante mortem and aqueous humor was collected 12 h post mortem from 63 sheep. Linear Regression Analysis was run to determine the equations predicting the serum value of each parameter based on the value determined in aqueous humor for those parameters that were significantly correlated. A strong relationship was found for Mg and creatinine concentration as well as for AST and GGT activities in aqueous humor and serum. Aqueous humor analysis of creatinine, Mg, AST and GGT levels can be used for the estimation of the corresponding concentration in blood, while, total proteins, albumin and Ca levels are of limited value. © 2018 Elsevier B.V.