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  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
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  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
  • View Item
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Cobalt induces hypoxia-inducible factor-1 alpha (HIF-1 alpha) in HeLa cells by an iron-independent, but ROS-, PI-3K- and MAPK-dependent mechanism

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Author
Triantafyllou, A.; Liakos, P.; Tsakalof, A.; Georgatsou, E.; Simos, G.; Bonanou, S.
Date
2006
DOI
10.1080/10715760600730810
Keyword
hypoxia
hypoxia-inducible factor-1
cobalt
iron
desferrioxamine
HeLa
cell proliferation
HYPOXIA-INDUCIBLE FACTOR
UBIQUITIN-PROTEASOME PATHWAY
TRANSCRIPTION
FACTORS
SIGNAL-TRANSDUCTION
FACTOR 1-ALPHA
HIF-ALPHA
TUMOR
ANGIOGENESIS
INDUCED APOPTOSIS
OXIDATIVE STRESS
GENE-EXPRESSION
Biochemistry & Molecular Biology
Metadata display
Abstract
The iron-chelator desferrioxamine (DFO) and the transition metal cobalt induce hypoxia-inducible factor-1 alpha (HIF-1 alpha) in normoxia. DFO stabilizes HIF-1 alpha from proteolysis by inhibiting the activity of iron-dependent prolyl hydroxylases, but the mechanism of action of cobalt is not fully elucidated. The purpose of this study was to examine the regulation of HIF-1 alpha induction and HeLa cell proliferation by cobalt and the role of iron in these processes. Our results show that, unlike DFO, induction of transcriptionally active HIF-1 alpha by CoCl2 cannot be abrogated by the addition of excess Fe3+, but involves the production of reactive oxygen species (ROS) and the operation of the phosphatidylinositol-3 kinase (PI-3K) and MAPK pathways. CoCl2, as well as DFO, decreased HeLa cell proliferation, but these effects were reversed by the addition of Fe3+. We conclude that the effect of cobalt on cell proliferation is iron-dependent, while its effects on HIF-1 alpha induction are ROS- and signaling pathways-dependent, but iron-independent.
URI
http://hdl.handle.net/11615/33713
Collections
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ. [19705]

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