dc.creator | Taitzoglou, I. A. | en |
dc.creator | Tsantarliotou, M. | en |
dc.creator | Kouretas, D. | en |
dc.creator | Kokolis, N. A. | en |
dc.date.accessioned | 2015-11-23T10:49:26Z | |
dc.date.available | 2015-11-23T10:49:26Z | |
dc.date.issued | 1999 | |
dc.identifier | 10.1046/j.1439-0272.1999.00294.x | |
dc.identifier.issn | 0303-4569 | |
dc.identifier.uri | http://hdl.handle.net/11615/33549 | |
dc.description.abstract | The effect of gossypol-a polycyclic compound isolated from cotton seeds-on the plasminogen activator activity of man and ram acrosomal extracts was explored in vitro. The action of gossypol on the plasminogen activator activity was investigated by a spectrophotometric method using the chromogenic substrate S-2251. Gossypol, a known antispermatogenic agent, was found to effectively inhibit human and ovine acrosomal plasminogen activator activity. The inhibition was dose-dependent. Plasminogen activator activity from man and ram extracts was completely inhibited by 350 mu mol l(-1) and 300 mu mol l(-1) of gossypol, respectively. In additional experiments, low, non-spermicidal concentrations of gossypol (2.5-40 mu mol l(-1)) were found to significantly inhibit plasmin activity in a dose-dependent manner. The results suggest that inhibition of both acrosomal plasminogen activator and plasmin activity is a possible mechanism by which gossypol exerts its antifertility effect, since the plasminogen activator/plasmin system plays a role in the whole process of ovum fertilization. | en |
dc.source | Andrologia | en |
dc.source.uri | <Go to ISI>://WOS:000084343600004 | |
dc.subject | gossypol | en |
dc.subject | plasminogen activator activity | en |
dc.subject | acrosomal enzymes | en |
dc.subject | man | en |
dc.subject | ram | en |
dc.subject | HUMAN-SPERMATOZOA | en |
dc.subject | SEMINAL PLASMA | en |
dc.subject | HYDROLYTIC ENZYMES | en |
dc.subject | SPERM | en |
dc.subject | RAM | en |
dc.subject | ACROSIN | en |
dc.subject | IDENTIFICATION | en |
dc.subject | TESTOSTERONE | en |
dc.subject | PENETRATION | en |
dc.subject | PROTEINASE | en |
dc.subject | Andrology | en |
dc.title | Gossypol-induced inhibition of plasminogen activator activity in human and ovine acrosomal extract | en |
dc.type | journalArticle | en |