Development and application of molecular markers for poultry meat identification in food chain

Abstract

Every year, large quantities of poultry and game meat are consumed. Thus, efficient techniques to identify the meat species origin are required which interest traders, consumers and organizations. In this study, two mitochondrial DNA (mtDNA) genes, Cytochrome b (Cyt b) and 12S ribosomal RNA (12S rRNA) were tested as putative discrimination markers in samples of raw and processed poultry meat (chicken, turkey, cluck, goose, pheasant, partridge, woodcock, ostrich, quail and song thrush), applying the PCR-RFLP technique with universal primers and ten different restriction enzymes. Digestion of 12S rRNA by Acil successfully distinguished all avian species, producing species-specific patterns. We conclude that the 12S rRNA gene is more informative than Cyt b gene for avian species identification purposes. Moderate process treatment did not prevent the species identification, presenting similar patterns with the raw meat. Finally, this method was considered sufficient to detect mixtures of meat, making it a valuable tool for checking possible adulterations. (C) 2010 Elsevier Ltd. All rights reserved.