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Biophysical characterization of V3-lipopeptide liposomes influencing HIV-1 infectivity
dc.creator | Rizos, A. K. | en |
dc.creator | Baritaki, S. | en |
dc.creator | Tsikalas, I. | en |
dc.creator | Doetschman, D. C. | en |
dc.creator | Spandidos, D. A. | en |
dc.creator | Krambovitis, E. | en |
dc.date.accessioned | 2015-11-23T10:46:29Z | |
dc.date.available | 2015-11-23T10:46:29Z | |
dc.date.issued | 2007 | |
dc.identifier | 10.1016/j.bbrc.2007.02.052 | |
dc.identifier.issn | 0006291X | |
dc.identifier.uri | http://hdl.handle.net/11615/32655 | |
dc.description.abstract | The V3-loop of the HIV-1 gp120 alters host cell immune function and modulates infectivity. We investigated biophysical parameters of liposome constructs with embedded lipopeptides from the principle neutralizing domain of the V3-loop and their influence on viral infectivity. Dynamic light scattering measurements showed liposome supramolecular structures with hydrodynamic radius of the order of 900 and 1300 nm for plain and V3-lipopeptide liposomes. Electron paramagnetic resonance measurements showed almost identical local microenvironment. The difference in liposome hydrodynamic radius was attributed to the fluctuating ionic environment of the V3-lipopeptide liposomes. In vitro HIV-1 infectivity assays showed that plain liposomes reduced virus production in all cell cultures, probably due to the hydrophobic nature of the aggregates. Liposomes carrying V3-lipopeptides with different cationic potentials restored and even enhanced infectivity (p < 0.05). These results highlight the need for elucidation of the involvement of lipid bilayers as dynamic components in supramolecular structures and in HIV-1 fusion mechanisms. © 2007 Elsevier Inc. All rights reserved. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-33847704102&partnerID=40&md5=b42287c63f95d07a77b61890efe4c0cf | |
dc.subject | Dynamic light scattering | en |
dc.subject | Electron paramagnetic resonance | en |
dc.subject | HIV infectivity | en |
dc.subject | Liposomes | en |
dc.subject | V3-lipopetides | en |
dc.subject | lipopeptide | en |
dc.subject | liposome | en |
dc.subject | third variable loop lipopeptide | en |
dc.subject | unclassified drug | en |
dc.subject | analytic method | en |
dc.subject | animal cell | en |
dc.subject | article | en |
dc.subject | cell culture | en |
dc.subject | controlled study | en |
dc.subject | electron spin resonance | en |
dc.subject | Human immunodeficiency virus 1 | en |
dc.subject | hydrodynamics | en |
dc.subject | light scattering | en |
dc.subject | nonhuman | en |
dc.subject | parameter | en |
dc.subject | priority journal | en |
dc.subject | protein analysis | en |
dc.subject | supramolecular chemistry | en |
dc.subject | virus infectivity | en |
dc.subject | Biophysics | en |
dc.subject | Electron Spin Resonance Spectroscopy | en |
dc.subject | HIV Core Protein p24 | en |
dc.subject | HIV Envelope Protein gp120 | en |
dc.subject | HIV-1 | en |
dc.subject | Humans | en |
dc.subject | Lipoproteins | en |
dc.subject | Peptides | en |
dc.title | Biophysical characterization of V3-lipopeptide liposomes influencing HIV-1 infectivity | en |
dc.type | journalArticle | en |
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