Age-dependent changes in 8-oxoguanine-DNA glycosylase activity are modulated by adaptive responses to physical exercise in human skeletal muscle
dc.creator | Radak, Z. | en |
dc.creator | Bori, Z. | en |
dc.creator | Koltai, E. | en |
dc.creator | Fatouros, I. G. | en |
dc.creator | Jamurtas, A. Z. | en |
dc.creator | Douroudos, II | en |
dc.creator | Terzis, G. | en |
dc.creator | Nikolaidis, M. G. | en |
dc.creator | Chatzinikolaou, A. | en |
dc.creator | Sovatzidis, A. | en |
dc.creator | Kumagai, S. | en |
dc.creator | Naito, H. | en |
dc.creator | Boldogh, I. | en |
dc.date.accessioned | 2015-11-23T10:46:17Z | |
dc.date.available | 2015-11-23T10:46:17Z | |
dc.date.issued | 2011 | |
dc.identifier | 10.1016/j.freeradbiomed.2011.04.018 | |
dc.identifier.issn | 0891-5849 | |
dc.identifier.uri | http://hdl.handle.net/11615/32563 | |
dc.description.abstract | 8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age. 8-OxoG levels in DNA were inversely correlated with the abundance of acetylated OGG1 (Ac-OGG1), but not with total OGG1, apurinic/apyrimidinic endonuclease 1 (APE1), or Ac-APE1. The actual Ac-OGG1 level was linked to exercise-induced oxidative stress, as shown by changes in lipid peroxide levels and expression of Cu,Zn-SOD, Mn-SOD, and SIRT3, as well as the balance between acetyltransferase p300/CBP and deacetylase SIRT1, but not SIRT6 expression. Together these data suggest that that acetylated form of OGG1, and not OGG1 itself, correlates inversely with the 8-oxoG level in the DNA of human skeletal muscle, and the Ac-OGG1 level is dependent on adaptive cellular responses to physical activity, but is age independent. (C) 2011 Elsevier Inc. All rights reserved. | en |
dc.source.uri | <Go to ISI>://WOS:000292352500017 | |
dc.subject | Exercise | en |
dc.subject | Aging | en |
dc.subject | DNA damage/repair | en |
dc.subject | Sirtuins | en |
dc.subject | Antioxidants | en |
dc.subject | 8-OxoG | en |
dc.subject | OGG1 | en |
dc.subject | Acetylation | en |
dc.subject | Free radicals | en |
dc.subject | BASE-EXCISION-REPAIR | en |
dc.subject | INDUCED OXIDATIVE STRESS | en |
dc.subject | DNA-DAMAGE | en |
dc.subject | FREE-RADICALS | en |
dc.subject | IN-VIVO | en |
dc.subject | AQUEOUS-SOLUTION | en |
dc.subject | AP-ENDONUCLEASE | en |
dc.subject | NUCLEAR-DNA | en |
dc.subject | CELL-DEATH | en |
dc.subject | MECHANISMS | en |
dc.subject | Biochemistry & Molecular Biology | en |
dc.subject | Endocrinology & Metabolism | en |
dc.title | Age-dependent changes in 8-oxoguanine-DNA glycosylase activity are modulated by adaptive responses to physical exercise in human skeletal muscle | en |
dc.type | journalArticle | en |
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