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dc.creatorPournaras, S.en
dc.creatorZarkotou, O.en
dc.creatorPoulou, A.en
dc.creatorKristo, I.en
dc.creatorVrioni, G.en
dc.creatorThemeli-Digalaki, K.en
dc.creatorTsakris, A.en
dc.date.accessioned2015-11-23T10:46:06Z
dc.date.available2015-11-23T10:46:06Z
dc.date.issued2013
dc.identifier10.1128/jcm.00901-13
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/11615/32479
dc.description.abstractCarbapenemase-producing Enterobacteriaceae (CPE) are rapidly spreading worldwide. Early detection of fecal CPE carriers is essential for effective infection control. Here, we evaluated the performance of a meropenem combined disk test (CDT) for rapidly differentiating CPE isolates directly from rectal swabs. The screening method was applied for 189 rectal swabs from hospitalized patients at high risk for CPE carriage. Swabs were suspended in 1 ml saline and cultured for confluent growth onto a MacConkey agar plate with a meropenem (MER) disk alone, a MER disk plus phenyl boronic acid (PBA), a MER disk plus EDTA, and a MER disk plus PBA and EDTA. An inhibition zone of <= 25 mm around the MER disk alone indicated carriage of carbapenem-resistant organisms. Furthermore, >= 5-mm differences in the inhibition zone between MER disks without and with the inhibitors (PBA, EDTA, or both) were considered positive results for detecting Klebsiella pneumoniae carbapenemase (KPC), metallo-beta-lactamase (MBL), or both carbapenemases, respectively. For comparison, rectal suspensions were tested using MacConkey plates with ertapenem (MacERT) disks and PCR (PCR-S) for carbapenemase genes. Of the 189 samples, 97 were genotypically confirmed as CPE positive by one of the three protocols tested. The CDT, MacERT disks, and PCR-S assays exhibited sensitivities of 94.8%, 96.9%, and 94.8% and specificities of 100%, 98.9%, and 100%, respectively, for detecting CPE-positive swabs. Moreover, the CDT correctly differentiated the production of KPC, MBL, or both carbapenemases in 78 of the 97 (80.4%) CPE-positive rectal swabs. Our results demonstrate that the CDT may provide a simple and inexpensive method for detecting and differentiating the carbapenemase type within a single day without requiring further testing and additional delay, supporting the timely implementation of infection control measures.en
dc.sourceJournal of Clinical Microbiologyen
dc.source.uri<Go to ISI>://WOS:000323214200028
dc.subjectRESISTANT KLEBSIELLA-PNEUMONIAEen
dc.subjectREAL-TIME PCRen
dc.subjectSCREENING AGAR PLATESen
dc.subjectINFECTION-CONTROLen
dc.subjectRAPID DETECTIONen
dc.subjectCHROMAGAR KPCen
dc.subjectSPREADen
dc.subjectMEDIAen
dc.subjectSPECIMENSen
dc.subjectBLA(KPC)en
dc.subjectMicrobiologyen
dc.titleA Combined Disk Test for Direct Differentiation of Carbapenemase-Producing Enterobacteriaceae in Surveillance Rectal Swabsen
dc.typejournalArticleen


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