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dc.creatorPetinaki, E.en
dc.creatorMiriagou, V.en
dc.creatorTzouvelekis, L. S.en
dc.creatorPournaras, S.en
dc.creatorHatzi, F.en
dc.creatorKontos, F.en
dc.creatorManiati, M.en
dc.creatorManiatis, A. N.en
dc.date.accessioned2015-11-23T10:45:26Z
dc.date.available2015-11-23T10:45:26Z
dc.date.issued2001
dc.identifier10.1016/S0924-8579(01)00327-2
dc.identifier.issn9248579
dc.identifier.urihttp://hdl.handle.net/11615/32183
dc.description.abstractA total of 250 consecutive Staphylococcus aureus clinical isolates were collected during the period 1999-2000 from the five major hospitals of the district of Thessaly (Central Greece). Thirty seven (14.8%) of the isolates were mecA-positive (MRSA) in a PCR-based assay; all exhibited resistance to oxacillin (agar dilution MICs ≥ 4 mg/L) and were also resistant to multiple antibiotics. Most of the MRSA isolates had been collected in the intensive care units and the surgical wards of the participating hospitals in a sporadic fashion. The MRSA incidence found here was significantly lower than reported in previous studies from Greece. Molecular typing by PFGE showed that the MRSA isolates were distributed between three pulsotypes. Evaluation of various conventional methods for assessing methicillin resistance showed that oxacillin agar dilution and immunological detection of PBP2a with the Slidex MRSA Detection kit were the most reliable in this setting. Misclassifications of isolates exhibiting low-level resistance (oxacillin MIC 2-4 mg/L) occurred with the salt agar screen, the oxacillin disk diffusion and the ATB Staph System methods. © 2001 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.en
dc.sourceInternational Journal of Antimicrobial Agentsen
dc.source.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-0034900096&partnerID=40&md5=109fa96a419b98e4228b67649007d0f5
dc.subjectMethicillin-resistanceen
dc.subjectStaphylococcus aureusen
dc.subjectagaren
dc.subjectbacterial proteinen
dc.subjectmeticillinen
dc.subjectoxacillinen
dc.subjectpenicillin binding protein 2aen
dc.subjectunclassified drugen
dc.subjectantibiotic resistanceen
dc.subjectarticleen
dc.subjectbacterium identificationen
dc.subjectbacterium isolateen
dc.subjectcontrolled studyen
dc.subjectdiffusionen
dc.subjectdilutionen
dc.subjectGreeceen
dc.subjecthospitalen
dc.subjectintensive care uniten
dc.subjectmethicillin resistant Staphylococcus aureusen
dc.subjectnonhumanen
dc.subjectpolymerase chain reactionen
dc.subjectpriority journalen
dc.subjectpulsed field gel electrophoresisen
dc.subjectStaphylococcus infectionen
dc.subjectstrain differenceen
dc.subjectsurgical warden
dc.subjectBacterial Proteinsen
dc.subjectCarrier Proteinsen
dc.subjectDrug Resistance, Multipleen
dc.subjectElectrophoresis, Gel, Pulsed-Fielden
dc.subjectHexosyltransferasesen
dc.subjectHospital Unitsen
dc.subjectHospitalsen
dc.subjectHumansen
dc.subjectIncidenceen
dc.subjectIntensive Care Unitsen
dc.subjectMethicillin Resistanceen
dc.subjectMicrobial Sensitivity Testsen
dc.subjectMuramoylpentapeptide Carboxypeptidaseen
dc.subjectPenicillin-Binding Proteinsen
dc.subjectPenicillinsen
dc.subjectPeptidyl Transferasesen
dc.subjectStaphylococcal Infectionsen
dc.subjectSurgery Department, Hospitalen
dc.titleMethicillin-resistant Staphylococcus aureus in the hospitals of Central Greeceen
dc.typejournalArticleen


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