Methicillin-resistant Staphylococcus aureus in the hospitals of Central Greece
AuthorPetinaki, E.; Miriagou, V.; Tzouvelekis, L. S.; Pournaras, S.; Hatzi, F.; Kontos, F.; Maniati, M.; Maniatis, A. N.
A total of 250 consecutive Staphylococcus aureus clinical isolates were collected during the period 1999-2000 from the five major hospitals of the district of Thessaly (Central Greece). Thirty seven (14.8%) of the isolates were mecA-positive (MRSA) in a PCR-based assay; all exhibited resistance to oxacillin (agar dilution MICs ≥ 4 mg/L) and were also resistant to multiple antibiotics. Most of the MRSA isolates had been collected in the intensive care units and the surgical wards of the participating hospitals in a sporadic fashion. The MRSA incidence found here was significantly lower than reported in previous studies from Greece. Molecular typing by PFGE showed that the MRSA isolates were distributed between three pulsotypes. Evaluation of various conventional methods for assessing methicillin resistance showed that oxacillin agar dilution and immunological detection of PBP2a with the Slidex MRSA Detection kit were the most reliable in this setting. Misclassifications of isolates exhibiting low-level resistance (oxacillin MIC 2-4 mg/L) occurred with the salt agar screen, the oxacillin disk diffusion and the ATB Staph System methods. © 2001 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.
Showing items related by title, author, creator and subject.
Predominance of a methicillin-resistant Staphylococcus aureus clone susceptible to erythromycin and several other non-β-lactam antibiotics in a Greek hospital Polyzou, A.; Slavakis, A.; Pournaras, S.; Maniatis, A. N.; Sofianou, D.; Tsakris, A. (2001)A clone of heterogeneously methicillin-resistant Staphylococcus aureus (MRSA) isolates susceptible to erythromycin, ciprofloxacin, clindamycin, co-trimoxazole, nitrofurantoin, rifampicin, tetracycline and vancomycin, ...
Evaluation of an anti-PBP 2a slide latex agglutination test in coagulase-negative staphylococci isolated in Greek hospitals Petinaki, E.; Miriagou, V.; Tzouvelekis, L. S.; Hatzi, F.; Legakis, N. J.; Maniatis, A. N. (2002)Oxacillin resistance was examined in 258 coagulase-negative staphylococci from Greek hospitals. mecA DNA was detected in 168 isolates, which were also resistant to oxacillin by agar dilution and disk diffusion, according ...
Dafopoulou, K.; Poulou, A.; Makris, D.; Hadjichristodoulou, C.; Tsakris, A.; Pournaras, S. (2013)The colistin resistance mechanisms in Acinetobacter baumannii are largely unknown. In the present study, we investigated the characteristics and the mechanisms of colistin resistance in two genetically indistinguishable ...