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dc.creatorPappa, A.en
dc.creatorSeferiadis, K.en
dc.creatorMarselos, M.en
dc.creatorTsolas, O.en
dc.creatorMessinis, I. E.en
dc.date.accessioned2015-11-23T10:44:47Z
dc.date.available2015-11-23T10:44:47Z
dc.date.issued1999
dc.identifier10.1016/s0093-691x(99)00038-2
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11615/32004
dc.description.abstractRat LH (rLH) and FSH (rFSH) were measured by sensitive and specific competition ELISAs. The rat LH ELISA used rLH-I-9 coated plates, an antiserum against rLH and an antibody against rabbit IgG labeled with peroxidase. Using rLH-RP-3 as a standard, rat LH was determined by binding of the anti-LH antibody to rLH-I-9 coated plates. The sensitivity of the assay was 0.8 ng/mL. Similarly, the rat FSH-ELISA used rFSH-I-8 coated plates, an antiserum against rFSH and an antibody against rabbit IgG labeled with peroxidase. Using rFSH-RP-3 as a standard, the FSH-ELISA was also determined by binding of the anti-FSH antibody to rFSH-I-8 coated plates. The sensitivity of this assay was 1.25 ng/mL. Both rat LH and FSH ELISA assays are highly specific and provide accurate determination of gonadotrophins in buffers, sera, cell culture media, and anterior pituitary extracts. These assays were used for monitoring the gonadotrophin surge-attenuating factor (GnSAF) and inhibin activities present in human follicular fluid (hFF). The 2 new ELISA procedures have practical advantages (safety, convenience, economy) over the RIA methods, and they perform as well as the RIA techniques at the same range of concentrations. (C) 1999 by Elsevier Science Inc.en
dc.source.uri<Go to ISI>://WOS:000080352900006
dc.subjectELISAen
dc.subjectrat LHen
dc.subjectrat FSHen
dc.subjectLINKED-IMMUNOSORBENT-ASSAYen
dc.subjectATTENUATING FACTOR GNSAFen
dc.subjectSURGE-INHIBITINGen
dc.subjectFACTORen
dc.subjectFOLLICLE-STIMULATING-HORMONEen
dc.subjectLUTEINIZING-HORMONEen
dc.subjectSUPEROVULATEDen
dc.subjectWOMENen
dc.subjectGONADOTROPINen
dc.subjectFLUIDen
dc.subjectSECRETIONen
dc.subjectIDENTIFICATIONen
dc.subjectReproductive Biologyen
dc.subjectVeterinary Sciencesen
dc.titleDevelopment and application of competitive ELISA assays for rat LH and FSHen
dc.typejournalArticleen


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