dc.creator | Papatheodorou, L. K. | en |
dc.creator | Malizos, K. N. | en |
dc.creator | Poultsides, L. A. | en |
dc.creator | Hantes, M. E. | en |
dc.creator | Grafanaki, K. | en |
dc.creator | Giannouli, S. | en |
dc.creator | Ioannou, M. G. | en |
dc.creator | Koukoulis, G. K. | en |
dc.creator | Protopappas, V. C. | en |
dc.creator | Fotiadis, D. I. | en |
dc.creator | Stathopoulos, C. | en |
dc.date.accessioned | 2015-11-23T10:44:30Z | |
dc.date.available | 2015-11-23T10:44:30Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1016/j.ultrasmedbio.2008.07.003 | |
dc.identifier.issn | 0301-5629 | |
dc.identifier.uri | http://hdl.handle.net/11615/31954 | |
dc.description.abstract | The present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) on the healing at tendon graft-bone interface, in molecular and histological level. The anterior cruciate ligament (ACL) in both knees of 52 New Zealand White rabbits was excised and replaced with the long digital extensor. A custom-made ultrasound transducer was implanted onto the medial tibial condyle, adjacent to the surface of the bone tunnel at both knees of the rabbits. The LiUS-treated right knees received 200-mu s bursts of 1 MHz sine waves at a pulse repetition rate of 1 kHz and with 30 MW/cm(2) spatial-average temporal-average intensity for 20 min daily (study group), while the left knee received no LiUS (control group). Thirty-six rabbits were used to perform semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis from both study and control groups for transforming growth factor-beta 1 (TGF-beta 1), biglycan and collagen I. RT-PCR products showed statistically significant upregulation of biglycan and collagen I gene expression in the study group, while TGF-beta 1 gene expression exhibited a bimodal profile. Histological examination performed in 16 rabbits from both groups supported the findings of the molecular analysis, indicating a faster healing rate and a more efficient ligamentization process after ultrasound treatment. These findings suggest that transosseous application of LiUS enhances the healing rate of the tendon graft-bone interface, possibly by affecting the expression levels of genes significant for the tendon to bone healing process. (E-mail: malizos@med.uth.gr) (c) 2009 World Federation for Ultrasound in Medicine & Biology. | en |
dc.source | Ultrasound in Medicine and Biology | en |
dc.source.uri | <Go to ISI>://WOS:000264977300007 | |
dc.subject | Low-intensity ultrasound (LiUS) | en |
dc.subject | Tendon graft-bone interface healing | en |
dc.subject | TGF-beta 1 | en |
dc.subject | Biglycan | en |
dc.subject | Collagen type I | en |
dc.subject | Anterior cruciate ligament (ACL) | en |
dc.subject | ANTERIOR CRUCIATE LIGAMENT | en |
dc.subject | MESSENGER-RNA LEVELS | en |
dc.subject | PULSED ULTRASOUND | en |
dc.subject | GROWTH-FACTORS | en |
dc.subject | PATELLAR TENDON | en |
dc.subject | TRANSFORMING GROWTH-FACTOR-BETA-1 | en |
dc.subject | AUTOGENOUS PATELLAR | en |
dc.subject | COLLAGEN-SYNTHESIS | en |
dc.subject | HUMAN-FIBROBLASTS | en |
dc.subject | MATRIX | en |
dc.subject | SYNTHESIS | en |
dc.subject | Acoustics | en |
dc.subject | Radiology, Nuclear Medicine & Medical Imaging | en |
dc.title | EFFECT OF TRANSOSSEOUS APPLICATION OF LOW-INTENSITY ULTRASOUND AT THE TENDON GRAFT-BONE INTERFACE HEALING: GENE EXPRESSION AND HISTOLOGICAL ANALYSIS IN RABBITS | en |
dc.type | journalArticle | en |