Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1
dc.creator | Noutsios, G. T. | en |
dc.creator | Papi, R. M. | en |
dc.creator | Ekateriniadou, L. V. | en |
dc.creator | Minas, A. | en |
dc.creator | Kyriakidis, D. A. | en |
dc.date.accessioned | 2015-11-23T10:41:23Z | |
dc.date.available | 2015-11-23T10:41:23Z | |
dc.date.issued | 2012 | |
dc.identifier | 10.1007/s11259-011-9505-7 | |
dc.identifier.issn | 1657380 | |
dc.identifier.uri | http://hdl.handle.net/11615/31425 | |
dc.description.abstract | In the present study forty-four Greek endemic strains of Br. melitensis and three reference strains were genotyped by Multi locus Variable Number Tandem Repeat (ML-VNTR) analysis based on an eight-base pair tandem repeat sequence that was revealed in eight loci of Br. melitensis genome. The forty-four strains were discriminated from the vaccine strain Rev-1 by Restriction Fragment Length Polymorphism (RFLP) and Denaturant Gradient Gel Electrophoresis (DGGE). The ML-VNTR analysis revealed that endemic, reference and vaccine strains are genetically closely related, while most of the loci tested (1, 2, 4, 5 and 7) are highly polymorphic with Hunter-Gaston Genetic Diversity Index (HGDI) values in the range of 0.939 to 0.775. Analysis of ML-VNTRs loci stability through in vitro passages proved that loci 1 and 5 are non stable. Therefore, vaccine strain can be discriminated from endemic strains by allele's clusters of loci 2, 4, 6 and 7. RFLP and DGGE were also employed to analyse omp2 gene and reveled different patterns among Rev-1 and endemic strains. In RFLP, Rev-1 revealed three fragments (282, 238 and 44 bp), while endemic strains two fragments (238 and 44 bp). As for DGGE, the electrophoretic mobility of Rev-1 is different from the endemic strains due to heterologous binding of DNA chains of omp2a and omp2b gene. Overall, our data show clearly that it is feasible to genotype endemic strains of Br. melitensis and differentiate them from vaccine strain Rev-1 with ML-VNTR, RFLP and DGGE techniques. These tools can be used for conventional investigations in brucellosis outbreaks. © 2011 Springer Science+Business Media B.V. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-84858703427&partnerID=40&md5=bd9ff2a3470e46723c697df7933d3198 | |
dc.subject | Brucella melitensis | en |
dc.subject | DGGE | en |
dc.subject | ML-VNTR analysis | en |
dc.subject | omp2 polymorphism | en |
dc.subject | Phylogenetic relationship | en |
dc.subject | Rev-1 | en |
dc.subject | Brucella vaccine | en |
dc.subject | DNA polymerase Rev1 | en |
dc.subject | outer membrane protein | en |
dc.subject | article | en |
dc.subject | bacterial genetics | en |
dc.subject | bacterial strain | en |
dc.subject | controlled study | en |
dc.subject | denaturing gradient gel electrophoresis | en |
dc.subject | electrophoretic mobility | en |
dc.subject | gene amplification | en |
dc.subject | gene frequency | en |
dc.subject | gene locus | en |
dc.subject | gene sequence | en |
dc.subject | genetic polymorphism | en |
dc.subject | genetic variability | en |
dc.subject | genotype | en |
dc.subject | geography | en |
dc.subject | Greece | en |
dc.subject | molecular typing | en |
dc.subject | nonhuman | en |
dc.subject | phylogeny | en |
dc.subject | polymerase chain reaction | en |
dc.subject | restriction fragment length polymorphism | en |
dc.subject | strain difference | en |
dc.subject | variable number of tandem repeat | en |
dc.subject | Animals | en |
dc.subject | Bacterial Proteins | en |
dc.subject | Bacterial Typing Techniques | en |
dc.subject | Bacterial Vaccines | en |
dc.subject | Base Sequence | en |
dc.subject | Brucellosis | en |
dc.subject | Cattle | en |
dc.subject | Cattle Diseases | en |
dc.subject | Deoxyribonucleases, Type II Site-Specific | en |
dc.subject | Minisatellite Repeats | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Multilocus Sequence Typing | en |
dc.subject | Polymorphism, Genetic | en |
dc.subject | Polymorphism, Restriction Fragment Length | en |
dc.subject | Porins | en |
dc.subject | Species Specificity | en |
dc.title | Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1 | en |
dc.type | journalArticle | en |
Αρχεία σε αυτό το τεκμήριο
Αρχεία | Μέγεθος | Τύπος | Προβολή |
---|---|---|---|
Δεν υπάρχουν αρχεία που να σχετίζονται με αυτό το τεκμήριο. |