Logo
    • English
    • Ελληνικά
    • Deutsch
    • français
    • italiano
    • español
  • English 
    • English
    • Ελληνικά
    • Deutsch
    • français
    • italiano
    • español
  • Login
View Item 
  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
  • View Item
  •   University of Thessaly Institutional Repository
  • Επιστημονικές Δημοσιεύσεις Μελών ΠΘ (ΕΔΠΘ)
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ.
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.
Institutional repository
All of DSpace
  • Communities & Collections
  • By Issue Date
  • Authors
  • Titles
  • Subjects

Sad3 and Sad4 are required for saponin biosynthesis and root development in oat

Thumbnail
Author
Mylona, P.; Owatworakit, A.; Papadopoulou, K.; Jenner, H.; Qin, B.; Findlay, K.; Hill, L.; Qi, X.; Bakht, S.; Melton, R.; Osbourn, A.
Date
2008
DOI
10.1105/tpc.107.056531
Keyword
Avena
avenacin A 1
saponin
sterol
vegetable protein
allele
article
biosynthesis
cell membrane
chemistry
cytology
fluorescence
gene dosage
genetics
metabolism
molecular genetics
mutation
oat
phenotype
plant epidermis
plant root
transmission electron microscopy
ultrastructure
Alleles
Avena sativa
Microscopy, Electron, Transmission
Molecular Sequence Data
Plant Proteins
Plant Roots
Saponins
Sterols
Metadata display
Abstract
Avenacins are antimicrobial triterpene glycosides that are produced by oat (Avena) roots. These compounds confer broad-spectrum resistance to soil pathogens. Avenacin A-1, the major avenacin produced by oats, is strongly UV fluorescent and accumulates in root epidermal cells. We previously defined nine loci required for avenacin synthesis, eight of which are clustered. Mutants affected at seven of these (including Saponin-deficient1 [Sad1], the gene for the first committed enzyme in the pathway) have normal root morphology but reduced root fluorescence. In this study, we focus on mutations at the other two loci, Sad3 (also within the gene cluster) and Sad4 (unlinked), which result in stunted root growth, membrane trafficking defects in the root epidermis, and root hair deficiency. While sad3 and sad4 mutants both accumulate the same intermediate, monodeglucosyl avenacin A-1, the effect on avenacin A-1 glucosylation in sad4 mutants is only partial. sad1/sad1 sad3/sad3 and sad1/sad1 sad4/sad4 double mutants have normal root morphology, implying that the accumulation of incompletely glucosylated avenacin A-1 disrupts membrane trafficking and causes degeneration of the epidermis, with consequential effects on root hair formation. Various lines of evidence indicate that these effects are dosage-dependent. The significance of these data for the evolution and maintenance of the avenacin gene cluster is discussed. © 2008 American Society of Plant Biologists.
URI
http://hdl.handle.net/11615/31215
Collections
  • Δημοσιεύσεις σε περιοδικά, συνέδρια, κεφάλαια βιβλίων κλπ. [19674]
Η δικτυακή πύλη της Ευρωπαϊκής Ένωσης
Ψηφιακή Ελλάδα
ΕΣΠΑ 2007-2013
Με τη συγχρηματοδότηση της Ελλάδας και της Ευρωπαϊκής Ένωσης
htmlmap 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister (MyDspace)
Help Contact
DepositionAboutHelpContact Us
Choose LanguageAll of DSpace
EnglishΕλληνικά
Η δικτυακή πύλη της Ευρωπαϊκής Ένωσης
Ψηφιακή Ελλάδα
ΕΣΠΑ 2007-2013
Με τη συγχρηματοδότηση της Ελλάδας και της Ευρωπαϊκής Ένωσης
htmlmap