Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression

Abstract

In the present study, the Sparus aurata alpha -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream a-actin is identical to Fugu alpha -actin-1. The expression of a-actin was initiated at the onset of segmentation. In adult fish, alpha -actin is expressed predominantly in white and red muscle. (C) 2001 Elsevier Science Inc. All rights reserved.