Regulation of aromatase expression by hormones, drugs pesticides and environmental pollutants in canine mammary CMT-U27 cells

Abstract

Aromatase, the enzyme that coverts testosterone to 17β-estradiol, has attracted attention lately because of its involvement in some forms of breast cancer in women. Studies are now undergoing on a variety of species to elucidate the effect of various agents on aromatase expression. Dog is an important species, widely used by the pharmaceutical industry for many study types, including those that will impact decision on drug development. In the present study, a wide variety of agents, including steroid hormones, drugs, pesticides, and environmental pollutants, were used to investigate their effect on aromatase expression in the canine mammary CMT-U27 cell line using quantitative real-time polymerase chain reaction (qPCR) on DNA synthesized from RNA extracted from the cells. ©PHARMAKON-Press.