Bone marrow side population cells are enriched for progenitors capable of myogenic differentiation
dc.creator | Luth, E. S. | en |
dc.creator | Jun, S. J. | en |
dc.creator | Wessen, M. K. | en |
dc.creator | Liadaki, K. | en |
dc.creator | Gussoni, E. | en |
dc.creator | Kunkel, L. M. | en |
dc.date.accessioned | 2015-11-23T10:38:14Z | |
dc.date.available | 2015-11-23T10:38:14Z | |
dc.date.issued | 2008 | |
dc.identifier | 10.1242/jcs.021675 | |
dc.identifier.issn | 219533 | |
dc.identifier.uri | http://hdl.handle.net/11615/30440 | |
dc.description.abstract | Although the contribution of bone marrow-derived cells to regenerating skeletal muscle has been repeatedly documented, there remains considerable debate as to whether this incorporation is exclusively a result of inflammatory cell fusion to regenerating myofibers or whether certain populations of bone marrow-derived cells have the capacity to differentiate into muscle. The present study uses a dual-marker approach in which GFP+ cells were intravenously transplanted into lethally irradiated β-galactosidase+ recipients to allow for simple determination of donor and host contribution to the muscle. FACS analysis of cardiotoxin-damaged muscle revealed that CD45+ bone-marrow side-population (SP) cells, a group enriched in hematopoietic stem cells, can give rise to CD45-/Sca1+/desmin+ cells capable of myogenic differentiation. Moreover, after immunohistochemical examination of the muscles of both SP- and whole bone marrow-transplanted animals, we noted the presence of myofibers composed only of bone marrow-derived cells. Our findings suggest that a subpopulation of bone marrow SP cells contains precursor cells whose progeny have the potential to differentiate towards a muscle lineage and are capable of de novo myogenesis following transplantation and initiation of muscle repair via chemical damage. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-46649095842&partnerID=40&md5=d18c004eb76c11c1f7269eaba897a1a4 | |
dc.subject | Bone marrow | en |
dc.subject | Progenitor | en |
dc.subject | Side population | en |
dc.subject | Skeletal muscle | en |
dc.subject | ataxin 1 | en |
dc.subject | beta galactosidase | en |
dc.subject | cardiotoxin | en |
dc.subject | CD45 antigen | en |
dc.subject | desmin | en |
dc.subject | MyoD protein | en |
dc.subject | transcription factor PAX7 | en |
dc.subject | animal cell | en |
dc.subject | animal tissue | en |
dc.subject | article | en |
dc.subject | bone marrow cell | en |
dc.subject | bone marrow transplantation | en |
dc.subject | cell differentiation | en |
dc.subject | cell lineage | en |
dc.subject | cell population | en |
dc.subject | controlled study | en |
dc.subject | female | en |
dc.subject | fluorescence activated cell sorting | en |
dc.subject | hematopoietic stem cell | en |
dc.subject | immunohistochemistry | en |
dc.subject | male | en |
dc.subject | mouse | en |
dc.subject | muscle development | en |
dc.subject | muscle fibril | en |
dc.subject | muscle injury | en |
dc.subject | muscle regeneration | en |
dc.subject | nonhuman | en |
dc.subject | priority journal | en |
dc.subject | stem cell | en |
dc.subject | Animals | en |
dc.subject | Antigens, CD45 | en |
dc.subject | beta-Galactosidase | en |
dc.subject | Biological Markers | en |
dc.subject | Bone Marrow Cells | en |
dc.subject | Cell Count | en |
dc.subject | Cell Separation | en |
dc.subject | Green Fluorescent Proteins | en |
dc.subject | Mice | en |
dc.subject | Muscle Fibers | en |
dc.subject | Nerve Tissue Proteins | en |
dc.subject | Nuclear Proteins | en |
dc.subject | Regeneration | en |
dc.subject | Stem Cells | en |
dc.subject | Animalia | en |
dc.title | Bone marrow side population cells are enriched for progenitors capable of myogenic differentiation | en |
dc.type | journalArticle | en |
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