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dc.creatorHavaki, S.en
dc.creatorKittas, C.en
dc.creatorMarinos, E.en
dc.creatorDafni, U.en
dc.creatorSotiropoulou, C.en
dc.creatorVoloudakis-Baltatzis, I.en
dc.creatorGoutas, N.en
dc.creatorVassilaros, S. D.en
dc.creatorAthanasiou, E.en
dc.creatorArvanitis, D. L.en
dc.date.accessioned2015-11-23T10:30:04Z
dc.date.available2015-11-23T10:30:04Z
dc.date.issued2003
dc.identifier10.1080/01913120390209875
dc.identifier.issn0191-3123
dc.identifier.urihttp://hdl.handle.net/11615/28481
dc.description.abstractThe monoclonal antibody H (mAbH) detects an epitope consisting of an O-linked N -acetyl glucosamine (O-GlcNAc) and neighboring amino acids. This epitope has been found by using extracts from the MCF-7 human breast carcinoma cell line in immunoblotting experiments, on cytokeratin 8 (CK8) and 5 other polypeptides. In the present study, a double immunogold method was applied for the colocalization of CK8 and mAbH epitope on epoxy thin sections in 18 cases of infiltrating ductal breast carcinomas (IDBC) and in 6 cases of fibroadenomas, to study the accurate subcellular distribution of CK8 in breast cancer cells, as compared to the 5 polypeptides, recognized by mAbH. Furthermore, a detailed quantitative evaluation of the double immunolocalization over the cellular compartments of cancer cells was undertaken with the aid of a computerized image analysis system and the results were assessed statistically. The distribution pattern of CK8 and the mAbH epitope in the neoplastic mammary epithelial cells was similar in IDBC as compared to fibroadenomas, while the gold labeling intensity of these epitopes differed over the cellular compartments between malignant and benign biopsies. The results reveal the significance of the role of CK8 and O-GlcNAc glycosylation in the biology of the neoplastic mammary cells in vivo, determining their malignant potential.en
dc.source.uri<Go to ISI>://WOS:000186931100003
dc.subjectantibody Hen
dc.subjectcytokeratin 8en
dc.subjectdouble immunogold labelingen
dc.subjectimage analysisen
dc.subjectinfiltrating ductal breast carcinomaen
dc.subjectultrastructureen
dc.subjectINTERMEDIATE-FILAMENT PROTEINSen
dc.subjectLINKED N-ACETYLGLUCOSAMINEen
dc.subjectTISSUEen
dc.subjectPOLYPEPTIDE ANTIGENen
dc.subjectMAMMARY EPITHELIAL-CELLSen
dc.subjectELECTRON-MICROSCOPYen
dc.subjectIMAGE-ANALYSISen
dc.subjectO-GLCNACen
dc.subjectENDOPLASMIC-RETICULUMen
dc.subjectCYTOSKELETAL PROTEINSen
dc.subjectCYTOPLASMIC PROTEINSen
dc.subjectMicroscopyen
dc.subjectPathologyen
dc.titleUltrastructural immunostaining of infiltrating ductal breast carcinomas with the monoclonal antibody H: A comparative study with cytokeratin 8en
dc.typejournalArticleen


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