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dc.creatorEleftheriadis, T.en
dc.creatorLiakopoulos, V.en
dc.creatorLawson, B.en
dc.creatorAntoniadi, G.en
dc.creatorStefanidis, I.en
dc.creatorGalaktidou, G.en
dc.date.accessioned2015-11-23T10:26:11Z
dc.date.available2015-11-23T10:26:11Z
dc.date.issued2011
dc.identifier.issn1108-4189
dc.identifier.urihttp://hdl.handle.net/11615/27323
dc.description.abstractBesides extracellular matrix production, fibroblasts are able to produce various cytokines. Their ubiquitous position makes fibroblasts appropriate cells for sensing various noxious stimuli and for attracting immune cells in the affected area. In the present study the effect of lipopolysaccharide (LPS) and cobalt chloride (CoCl(2)) on the above fibroblasts functions were evaluated in primary human skin fibroblasts cultures. Collagen, matrix metalloproteinase-1, tissue inhibitor of metalloproteinases-1, transforming growth factor-beta 1, interleukin-8 (IL-8) and macrophage chemoattractant protein-1 (MCP-1) were measured in fibroblasts culture supernatants. Fibroblasts proliferation and viability were assessed as well. Hypoxia inducible factor-1 alpha and the phosphorylated p65 portion of NF-kappa B were assessed in fibroblasts protein extracts. LPS and CoCl(2) had a minor effect on fibrosis related factors in human primary fibroblasts, possibly due to the absence of interplay with other cell types in the used experimental system. On the contrary both LPS and CoCl(2) increased significantly IL-8. LPS also increased considerably MCP-1, but CoCl(2) decreased it. Thus LPS and CoCl(2) induce a sentinel, nevertheless not identical, phenotype in primary human fibroblasts. The last disparity could result in different body response to infectious or hypoxic noxious stimuli. Hippokratia 2011; 15 (3): 238-243en
dc.source.uri<Go to ISI>://WOS:000293229100007
dc.subjectfibroblasten
dc.subjecthypoxiaen
dc.subjectinfectionen
dc.subjectfibrosisen
dc.subjectchemokinesen
dc.subjectlipopolysaccharideen
dc.subjectcobalt chlorideen
dc.subjectmonocyte chemoattractant protein-1en
dc.subjectinterlukin-8en
dc.subjectNF-KAPPA-Ben
dc.subjectNEUTROPHIL CHEMOTACTIC FACTORen
dc.subjectSYNOVIAL FIBROBLASTSen
dc.subjectINDUCIBLE FACTOR-1en
dc.subjectMATRIX-METALLOPROTEINASEen
dc.subjectIMMUNE-RESPONSESen
dc.subjectINNATEen
dc.subjectIMMUNITYen
dc.subjectEXPRESSIONen
dc.subjectRECEPTORen
dc.subjectBETAen
dc.subjectMedicine, General & Internalen
dc.titleLipopolysaccharide and hypoxia significantly alters interleukin-8 and macrophage chemoattractant protein-1 production by human fibroblasts but not fibrosis related factorsen
dc.typejournalArticleen


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