A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758)

Abstract

Optimal conditions of short-term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution (1:3) and incubated for 1h in acidic, pH 6.5 (AC group) or alkaline, pH 8.5 (AL group) conditions. Subsequently, the sperm of both groups was further diluted (1:3) in the immobilizing solution and the pH was adjusted to pH 7.51 (+/- 0.02) and 7.56 (+/- 0.07) in the AC and AL group respectively. The samples were kept in sealed bags and stored at 4 degrees C. Viability (%) and motility (%) was estimated in samples obtained daily for the next 4days. The results indicate that even a brief exposure of sperm to acid acidic conditions prior to storage accelerated the decline of sperm viability and motility during refrigeration.